1887

Abstract

Two Gram-negative, non-motile, non-spore-forming, moderately halophilic strains, JG-100 and JG-102, were isolated from squid jeotgal, a traditional Korean fermented seafood. The two strains grew optimally at 30 °C and in the presence of 2–3 % (v/w) NaCl. Strains JG-100 and JG-102 were characterized chemotaxonomically; they both had ubiquinone-8 as the predominant respiratory lipoquinone and C 9 as the major fatty acid. Their DNA G+C content was 44 mol%. Strains JG-100 and JG-102 showed 1 bp difference in their 16S rRNA gene sequences and a mean DNA–DNA relatedness level of 88 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains JG-100 and JG-102 form a distinct phylogenetic lineage within the cluster comprising species. The 16S rRNA gene sequences of strains JG-100 and JG-102 had similarity levels of 95·2–98·4 % to sequences of the type strains of recognized species. Levels of DNA–DNA relatedness between strains JG-100 and JG-102 and the type strains of some phylogenetically related species were 6–24 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains JG-100 and JG-102 should be placed in the genus as a novel species, for which the name sp. nov. (type strain, JG-100=KCTC 12186=DSM 16065) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63140-0
2005-01-01
2021-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550171.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63140-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K. 1996; Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46:841–848 [CrossRef]
    [Google Scholar]
  2. Bowman J. P., Nichols D. S., McMeekin T. A. 1997; Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst Appl Microbiol 20:209–215 [CrossRef]
    [Google Scholar]
  3. Bozal N., Montes M. J., Tudela E., Guinea J. 2003; Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp.nov. and Psychrobacter fozii sp. nov.. Int J Syst Evol Microbiol 53:1093–1100 [CrossRef]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Denner E. B. M., Mark B., Busse H.-J., Turkiewicz M., Lubitz W. 2001; Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease. Syst Appl Microbiol 24:44–53 [CrossRef]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Juni E. 1991; The genus Psychrobacter . In The Prokaryotes pp  3241–3246 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  9. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Albrecht A., Buczolits S., Busse H.-J. 2002; Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25:31–36 [CrossRef]
    [Google Scholar]
  11. Komagata K., Suzuki K. I. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  12. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Levring T. 1946; Some culture experiments with Ulva and artificial seawater. K Fysiogr Sallsk Lund Forh 16:45–56
    [Google Scholar]
  15. Maruyama A., Honda D., Yamamoto H., Kitamura K., Higashihara T. 2000; Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50:835–846 [CrossRef]
    [Google Scholar]
  16. Romanenko L. A., Schumann P., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2002; Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles isolated from marine environments. Int J Syst Evol Microbiol 52:1291–1297 [CrossRef]
    [Google Scholar]
  17. Romanenko L. A., Lysenko A. M., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54:1741–1745 [CrossRef]
    [Google Scholar]
  18. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  19. Shaw B. G., Latty J. B. 1988; A numerical taxonomic study of non-motile non-fermentative Gram-negative bacteria from foods. J Appl Bacteriol 65:7–21 [CrossRef]
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Vela A. I., Collins M. D., Latre M. V., Mateos A., Moreno M. A., Hutson R., Domínguez L., Fernández-Garayzábal J. F. 2003; Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int J Syst Evol Microbiol 53:415–419 [CrossRef]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  27. Yumoto I., Hirota K., Sogabe Y., Nodasaka Y., Yokota Y., Hoshino T. 2003; Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 53:1985–1989 [CrossRef]
    [Google Scholar]
  28. Yurkov V., Stackebrandt E., Holmes A. 7 other authors 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a -containing bacteria and description of Roseococcus thiosulfatophilus gen.nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–434 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63140-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63140-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error