@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63130-0, author = "Yumoto, Isao and Hirota, Kikue and Nodasaka, Yoshinobu and Yokota, Yuji and Hoshino, Tamotsu and Nakajima, Kenji", title = "Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2004", volume = "54", number = "6", pages = "2379-2383", doi = "https://doi.org/10.1099/ijs.0.63130-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63130-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A psychrotolerant, obligately alkaliphilic bacterium, IDR2-2T, which is able to reduce indigo dye was isolated from a fermented polygonum indigo (Polygonum tinctorium Lour.) produced in Date, Hokkaido, using a traditional Japanese method. The isolate grew at pH 9–12 but not at pH 7–8. It was a Gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. The isolate grew in 0–17 % (w/v) NaCl but not at NaCl concentrations higher than 18 % (w/v). Its major cellular fatty acids were C14 : 0, C16 : 0, C16 : 19c and C18 : 19c, and its DNA G+C content was 40·6 mol%. dl-lactic acid was the major end-product from d-glucose. No quinones could be detected. The peptidoglycan type was A4β, Orn–d-Glu. A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain IDR2-2T is a member of the genus Alkalibacterium. DNA–DNA hybridization revealed low relatedness (less than 25 %) between the isolate and two phylogenetically related strains, Alkalibacterium olivapovliticus and Marinilactibacillus psychrotolerans. On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate merits classification as a novel species, for which the name Alkalibacterium psychrotolerans sp. nov. is proposed. The type strain is IDR2-2T (=JCM 12281T=NCIMB 13981T).", }