1887

Abstract

A psychrotolerant, obligately alkaliphilic bacterium, IDR2-2, which is able to reduce indigo dye was isolated from a fermented polygonum indigo ( Lour.) produced in Date, Hokkaido, using a traditional Japanese method. The isolate grew at pH 9–12 but not at pH 7–8. It was a Gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. The isolate grew in 0–17 % (w/v) NaCl but not at NaCl concentrations higher than 18 % (w/v). Its major cellular fatty acids were C, C, C9 and C9, and its DNA G+C content was 40·6 mol%. -lactic acid was the major end-product from -glucose. No quinones could be detected. The peptidoglycan type was A4, Orn–-Glu. A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain IDR2-2 is a member of the genus . DNA–DNA hybridization revealed low relatedness (less than 25 %) between the isolate and two phylogenetically related strains, and . On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate merits classification as a novel species, for which the name sp. nov. is proposed. The type strain is IDR2-2 (=JCM 12281=NCIMB 13981).

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2004-11-01
2020-09-25
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