1887

Abstract

The characterization of a novel slowly growing, scotochromogenic species is reported. This previously undescribed mycobacterial species was isolated from five different patients with symptomatic pulmonary infections. All isolates were acid-fast-positive and the mycolic acid profiles were unique and supported placement into the genus . Phenotypic characteristics of each strain included optimal growth after 3 weeks at a temperature range of 30–35 °C, yellow pigmentation after incubation in the dark and production of a heat-stable catalase. The 16S rRNA gene and internal transcribed spacer 1 sequences were identical for all five strains, but distinct from all known mycobacterial species. Phylogenetic analysis based on the 16S rRNA gene sequence placed the novel species within the slowly growing mycobacteria group in close proximity to , , and . These data support the conclusion that the related five described organisms represent a novel species, for which the name sp. nov. is proposed, with the type strain UNMC-MY1349 (=ATCC BAA-837=DSM 44803).

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2004-11-01
2019-10-19
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Alignment of hypervariable regions A and B of 16S rRNA gene sequences (a) and the hypervariable region of ITS1 (b) from selected slowly growing species. Sequences of were used as the reference sequences. Positions are defined in reference to the 16S rRNA gene sequence of . Only nucleotides that differ from are shown, while dashes indicate deletions. [PDF](49 KB)

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Expanded phylogenetic tree of selected species using the entire 16S rRNA gene sequence. The tree was constructed using the neighbour-joining method and rooted with as an outgroup. GenBank accession numbers are shown in parentheses. [PDF](17 KB)

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