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Volume 55, Issue 1

Emended description of porcine [] , [] and [] Free

Abstract

The aim of this study was to improve the definition and identification of a group of veterinarily important bacteria referred to as the [] –[] –[] complex. These organisms have mainly been isolated from the reproductive and intestinal tracts of pigs and in most cases have been considered as opportunistic pathogens. A collection of 87 strains were characterized by phenotypic analysis from which 41 strains were selected for 16S rRNA gene sequence comparison, out of which 23 have been sequenced in the present study. One group of 21 strains phenotyped as biovars 1, 3–5, 9–11, 19 and 25–27, including the type strain of [] , showed 16S rRNA gene sequence similarities of 99·6 % or higher; another group of 18 strains including biovars 2, 6–8, 12–15, 21, 23, 24 and 26A and the type strain of [] showed 97·8 % or higher 16S rRNA gene sequence similarity. Between the two groups, 93·8–95·7 % 16S rRNA gene sequence similarity was observed. Strains of [] showed 99·5 % similarity, with 95·5–97·2 and 93·8–95·5 % similarity to strains of [] and [] , respectively. Four strains could not be classified with any of these groups and belonged to other members of . Comparisons were also made to DNA–DNA hybridization data. Biovars 1, 9, 10, 11 and 19, including the type strain of [] , linked at 70 % DNA reassociation, whereas strains identified as biovars 2, 6, 7, 8, 12, 15 and 21 of [] linked at 81 %. The latter group most likely represents [] based on the 16S rRNA gene sequence comparisons. DNA reassociation between the [] and [] groups was at most 37 %, whereas 47 % was the highest DNA reassociation found between [] and [] . The study showed that [] , [] and [] can not be easily separated and may consequently be misidentified based on current knowledge of their phenotypic characteristics. In addition, these taxa are difficult to separate from other taxa of the . A revised scheme for separation based upon phenotypic characters is suggested for the three species [] emend., [] emend. and [] emend., with the respective type strains ATCC 27883, NCTC 10699 and ATCC 27072.

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2005-01-01
2024-04-18
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Emended description of porcine [Pasteurella] aerogenes, [Pasteurella] mairii and [Actinobacillus] rossii
Int J Syst Evol Microbiol 55, 209 (2005); https://doi.org/10.1099/ijs.0.63119-0
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