1887

Abstract

Dermatitis digitalis is an economically important ulcerative disease of undetermined aetiology affecting the hooves of cattle. Material was examined from two independent cases of this disease in Switzerland. Cultures from the advancing front of both lesions yielded large numbers of closely related short, mesophilic, non-motile, non-spore-forming, anaerobic, proteolytic, Gram-positive rods. The 16S rRNA gene sequences of strains OMZ 913 and OMZ 915 were identical and indicate and as their closest relatives. Phenotypically, the novel isolates are clearly distinguished from related bacteria by protein and antigen patterns, by cellular fatty acids and by API ZYM activities. The diamino acid of the Gram-positive cell wall is ornithine and the G+C content of OMZ 913 DNA is 44·4 mol%. The phylogenetic distance from recognized taxa in the phylum is sufficient to place these bovine isolates into a novel genus and species, for which the name gen. nov., sp. nov. is proposed, with OMZ 913 (=CIP 108087=DSM 15657) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63116-0
2005-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550667.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63116-0&mimeType=html&fmt=ahah

References

  1. Bickar D., Reid P. D. 1992; A high-affinity protein stain for Western blots, tissue prints, and electrophoretic gels. Anal Biochem 203:109–115 [CrossRef]
    [Google Scholar]
  2. Blowey R. W., Sharp M. W. 1988; Digital dermatitis in dairy cattle. Vet Rec 122:505–508 [CrossRef]
    [Google Scholar]
  3. Cato E. P., Moore L. V. H., Moore W. E. C. 1985; Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus. Int J Syst Bacteriol 35:475–477 [CrossRef]
    [Google Scholar]
  4. Choi B.-K., Nattermann H., Grund S., Haider W., Göbel U. B. 1997; Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis. Int J Syst Bacteriol 47:175–181 [CrossRef]
    [Google Scholar]
  5. Cole J. R., Chai B., Marsh T. L. 8 other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  6. Collighan R. J., Woodward M. J. 1997; Spirochaetes and other bacterial species associated with bovine digital dermatitis. FEMS Microbiol Lett 156:37–41 [CrossRef]
    [Google Scholar]
  7. Collighan R. J., Naylor R. D., Martin P. K., Cooly B. A., Buller N., Woodward M. J. 2000; A spirochete isolated from a case of severe virulent ovine foot disease is closely related to a treponeme isolated from human periodontitis and bovine digital dermatitis. Vet Microbiol 74:249–257 [CrossRef]
    [Google Scholar]
  8. Demirkan I., Carter S. D., Murray R. D., Blowey R. W., Woodward M. J. 1998; The frequent detection of a treponeme in bovine digital dermatitis by immunocytochemistry and polymerase chain reaction. Vet Microbiol 60:285–292 [CrossRef]
    [Google Scholar]
  9. Demirkan I., Carter S. D., Hart C. A., Woodward M. J. 1999; Isolation and cultivation of a spirochaete from bovine digital dermatitis. Vet Rec 145:497–498 [CrossRef]
    [Google Scholar]
  10. Dewhirst F. E., Chien C. C., Paster B. J., Ericson R. L., Orcutt R. P., Schauer D. B., Fox J. G. 1999; Phylogeny of the defined murine microbiota: altered Schaedler flora. Appl Environ Microbiol 65:3287–3292
    [Google Scholar]
  11. Döpfer D., Koopmans A., Meijer F. A. 7 other authors 1997; Histological and bacteriological evaluation of digital dermatitis in cattle, with special reference to spirochaetes and Campylobacter faecalis . Vet Rec 140:620–623 [CrossRef]
    [Google Scholar]
  12. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  13. Holdeman L. V., Moore W. E. C. 1975 Anaerobe Laboratory Manual Blacksburg, VA: VPI Anaerobe Laboratory;
    [Google Scholar]
  14. Kevbrin V. V., Zhilina T. N., Rainey F. A., Zavarzin G. A. 1998; Tindallia magadii gen. nov., sp. nov.: an alkaliphilic anaerobic ammonifier from soda lake deposits. Curr Microbiol 37:94–100 [CrossRef]
    [Google Scholar]
  15. Laven R. 2001; Control of digital dermatitis in cattle. In Practice 23:336–341 [CrossRef]
    [Google Scholar]
  16. Loesche W. J., Hockett R. N., Syed S. A. 1972; The predominant cultivable flora of tooth surface plaque removed from institutionalized subjects. Arch Oral Biol 17:1311–1325 [CrossRef]
    [Google Scholar]
  17. Luginbühl A., Kollbrunner M. 2000; Vermehrtes Auftreten der Dermatitis Digitalis des Rindes (Erdbeerfuss) in der Schweiz (Increasing number of cattle suffering from papillomatous digital dermatitis in Switzerland). Schweiz Arch Tierh 142:513–519 (in German
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Moter A., Leist G., Rudolph R., Schrank K., Choi B.-K., Wagner M., Göbel U. B. 1998; Fluorescence in situ hybridization shows spatial distribution of as yet uncultured treponemes in biopsies from digital dermatitis lesions. Microbiology 144:2459–2467 [CrossRef]
    [Google Scholar]
  20. Nakazawa F., Sato M., Poco S. E., Hashimura T., Ikeda T., Kalfas S., Sundqvist G., Hoshino E. 2000; Description of Mogibacterium pumilum gen.nov., sp. nov. and Mogibacterium vescum gen. nov., sp. nov., and reclassification of Eubacterium timidum (Holdeman et al . 1980) as Mogibacterium timidum gen. nov., comb. nov. Int J Syst Evol Microbiol 50:679–688 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of the bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  23. Schrank K., Choi B.-K., Grund S., Moter A., Heuner K., Nattermann H., Göbel U. B. 1999; Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. Int J Syst Bacteriol 49:43–50 [CrossRef]
    [Google Scholar]
  24. Shibahara T., Ohya T., Ogihara Y., Ishikawa Y., Kadota K. 2002; Concurrent spirochaetal infections of the feet and colon of cattle in Japan. Aust Vet J 80:497–502 [CrossRef]
    [Google Scholar]
  25. Stamm L. V., Bergen H. L., Walker R. L. 2002; Molecular typing of papillomatous digital dermatitis-associated Treponema isolates based on analysis of 16S-23S ribosomal DNA intergenic spacer regions. J Clin Microbiol 40:3463–3469 [CrossRef]
    [Google Scholar]
  26. Uematsu H., Nakazawa F., Ikeda T., Hoshino E. 1993; Eubacterium saphenum sp. nov., isolated from human periodontal pockets. Int J Syst Bacteriol 43:302–304 [CrossRef]
    [Google Scholar]
  27. Van De Peer Y., De Wachter R. 1993; treecon: a software package for the construction and drawing of evolutionary trees. Comput Appl Biosci 9:177–182
    [Google Scholar]
  28. Walker R. L., Read D. H., Loretz K. J., Nordhausen R. W. 1995; Spirochetes isolated from dairy cattle with papillomatous digital dermatitis and interdigital dermatitis. Vet Microbiol 47:343–355 [CrossRef]
    [Google Scholar]
  29. Wyss C. 1998; Flagellins, but not endoflagellar sheath proteins, of Treponema pallidum and of pathogen-related oral spirochetes are glycosylated. Infect Immun 66:5751–5754
    [Google Scholar]
  30. Wyss C., Choi B.-K., Schüpbach P., Guggenheim B., Göbel U. B. 1996; Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46:745–752 [CrossRef]
    [Google Scholar]
  31. Wyss C., Dewhirst F. E., Gmür R., Thurnheer T., Xue Y., Schüpbach P., Guggenheim B., Paster B. J. 2001; Treponema parvum sp. nov., a small glucuronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 51:955–962 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63116-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63116-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error