1887

Abstract

Dermatitis digitalis is an economically important ulcerative disease of undetermined aetiology affecting the hooves of cattle. Material was examined from two independent cases of this disease in Switzerland. Cultures from the advancing front of both lesions yielded large numbers of closely related short, mesophilic, non-motile, non-spore-forming, anaerobic, proteolytic, Gram-positive rods. The 16S rRNA gene sequences of strains OMZ 913 and OMZ 915 were identical and indicate and as their closest relatives. Phenotypically, the novel isolates are clearly distinguished from related bacteria by protein and antigen patterns, by cellular fatty acids and by API ZYM activities. The diamino acid of the Gram-positive cell wall is ornithine and the G+C content of OMZ 913 DNA is 44·4 mol%. The phylogenetic distance from recognized taxa in the phylum is sufficient to place these bovine isolates into a novel genus and species, for which the name gen. nov., sp. nov. is proposed, with OMZ 913 (=CIP 108087=DSM 15657) as the type strain.

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2005-03-01
2019-10-22
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Transmission electron micrograph of cells of strain OMZ 913 . Bacteria were fixed in cacodylate buffer with 2.5 % glutaraldehyde and 1 % OsO and stained with 1 % uranyl acetate; Epon sections were contrasted with lead citrate. Bar, 200 nm.

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SDS-PAGE of cellular extracts of novel dermatitis digitalis isolates and representative phylogenetic relatives blotted onto nitrocellulose and stained for protein by the reversible copper phthalocyanine tetrasulphonic acid method (CPTS; Bickar & Reid, 1992). Lanes: 1, OMZ 915; 2, OMZ 913 ; 3, DSM 10318 ; 4, ATCC 49989 ; 5, ATCC 35585 ; 6, D6B.23; 7, ATCC 33099 . The position of molecular mass markers is indicated on the margin in kDa.

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