1887

Abstract

Isolates 302, 334, 356, 377 and 379, detected in modified-atmosphere-packaged broiler meat, together with strains LMG 12317 and LMG 13617, detected in dog tonsils, were analysed in a polyphasic taxonomy study, including numerical analysis of ribopatterns and whole-cell protein patterns, 16S rRNA gene sequence analysis, DNA–DNA hybridization and determination of some phenotypic properties. The results indicated that these isolates represent a novel species in the genus . The isolates showed classical phenotypic reactions for the genus with the exception of not possessing the Lancefield group D antigen. Isolates 334, LMG 12317 and LMG 13617 showed the highest 16S rRNA gene sequence similarity (98·3–99·0 %) to the type strain. In the distance matrix tree based on 16S rRNA gene sequences, the three isolates were located in the group with as their closest phylogenetic neighbour. Numerical analyses of whole-cell protein patterns and dIII/RI ribotypes placed all seven isolates together in a single cluster separated from the group reference strains. The DNA–DNA hybridization level between strains 334 and LMG 12317 was 93·5 %, confirming that they represent the same species. Low hybridization levels (12–30 %) were, by contrast, obtained with the and type strains. The name sp. nov. is proposed, with strain LMG 12317 (=CCUG 48100) as the type strain.

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2004-09-01
2019-10-22
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Transmission electron micrograph of negatively stained (1 % phosphotungstic acid) cells of sp. nov. LMG 12317 .

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