1887

Abstract

Two novel bacteria, with an optimum growth temperature of approximately 60 °C, were isolated from Lu-shan hot springs in the central region of Taiwan. These isolates were aerobic, thermophilic, halotolerant, pink-pigmented, heterotrophic and resistant to gamma-radiation. Both pleomorphic, short, rod-shaped cells and coccoid cells were observed. Strains LS-286 (=ATCC BAA-452=BCRC 17198) and LS-293 (=ATCC BAA-406=BCRC 17173) represented a novel species of the genus , according to a phylogenetic analysis of the 16S rRNA gene, DNA–DNA hybridization, biochemical features and fatty acid composition. The name sp. nov. is proposed for this novel species, with LS-293 as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63109-0
2004-09-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541849.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63109-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Carreto L., Moore E., Nobre M. F., Wait R., Riley P. W., Sharp R. J., da Costa M. S. 1996; Rubrobacter xylanophilus sp. nov., a new thermophilic species isolated from a thermally polluted effluent. Int J Syst Bacteriol 46:460–465 [CrossRef]
    [Google Scholar]
  3. Chen M.-Y., Lin G.-H., Lin Y.-T., Tsay S.-S. 2002a; Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan. Int J Syst Evol Microbiol 52:1647–1654 [CrossRef]
    [Google Scholar]
  4. Chen M.-Y., Tsay S.-S., Chen K.-Y., Shi Y.-C., Lin Y.-T., Lin G.-H. 2002b; Pseudoxanthomonas taiwanensis sp. nov., a novel thermophilic, N2O-producing species isolated from hot springs. Int J Syst Evol Microbiol 52:2155–2161 [CrossRef]
    [Google Scholar]
  5. Chung A. P., Rainey F., Nobre M. F., Burghardt J., da Costa M. S. 1997; Meiothermus cerbereus sp. nov., a new slightly thermophilic species with high levels of 3-hydroxy fatty acids. Int J Syst Bacteriol 47:1225–1230 [CrossRef]
    [Google Scholar]
  6. Degryse E., Glansdorff N., Pierard A. 1978; A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus . Arch Microbiol 117:189–196 [CrossRef]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [CrossRef]
    [Google Scholar]
  9. Ferreira A. C., Nobre M. F., Moore E., Rainey F. A., Battista J. R., da Costa M. S. 1999; Characterization and radiation resistance of new isolates of Rubrobacter radiotolerans and Rubrobacter xylanophilus . Extremophiles 3:235–238 [CrossRef]
    [Google Scholar]
  10. Garrity G. M., Holt J. G. 2001; Phylum BVII. Thermomicrobia phy. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1p– 447 Edited by Boone D. R., Castenholz R. W. New York: Springer;
    [Google Scholar]
  11. Green P. N., Bousfield I. J. 1983; Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito and Iizuka 1971) comb. nov., corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov. Int J Syst Bacteriol 33:875–877 [CrossRef]
    [Google Scholar]
  12. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  13. Hansma H. G., Hoh J. H. 1994; Biomolecular imaging with the atomic force microscope. Annu Rev Biophys Biomol Struct 23:115–139 [CrossRef]
    [Google Scholar]
  14. Harvey D. J. 1982; Picolinyl esters as derivatives for the structural determination of long chain branched and unsaturated fatty acids. Biomed Mass Spectrom 9:33–38 [CrossRef]
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  16. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  17. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  18. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487 [CrossRef]
    [Google Scholar]
  19. Manaia C. M., da Costa M. S. 1991; Characterization of halotolerant Thermus isolates from shallow marine hot springs on S. Miguel, Azores. J Gen Microbiol 137:2643–2648 [CrossRef]
    [Google Scholar]
  20. Mattimore V., Battista J. R. 1996; Radioresistance of Deinococcus radiodurans : functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637
    [Google Scholar]
  21. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  22. Moseley B. E. B. 1967; The isolation and some properties of radiation-sensitive mutants of Micrococcus radiodurans . J Gen Microbiol 49:293–300 [CrossRef]
    [Google Scholar]
  23. Moseley B. E. B., Evans D. M. 1983; Isolation and properties of strains of Micrococcus ( Deinococcus ) radiodurans unable to excise ultraviolet light-induced pyrimidine dimers from DNA: evidence for two excision pathways. J Gen Microbiol 129:2437–2445
    [Google Scholar]
  24. Moseley B. E., Mattingly A. 1971; Repair of irradiation transforming deoxyribonucleic acid in wild type and a radiation-sensitive mutant of Micrococcus radiodurans . J Bacteriol 105:976–983
    [Google Scholar]
  25. Nishimura Y., Ino T., Iizuka H. 1988; Acinetobacter radioresistens sp. nov. isolated from cotton and soil. Int J Syst Bacteriol 38:209–211 [CrossRef]
    [Google Scholar]
  26. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  27. Pond J. L., Langworthy T. A., Holzer G. 1986; Long-chain diols : a new class of membrane lipids from a thermophilic bacterium. Science 231:1134–1136 [CrossRef]
    [Google Scholar]
  28. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  29. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  30. Sanders S. W., Maxcy R. B. 1979; Patterns of cell division, DNA base compositions, and fine structures of some radiation-resistant vegetative bacteria found in food. Appl Environ Microbiol 37:159–168
    [Google Scholar]
  31. Santos M. A., Williams R. A. D., da Costa M. S. 1989; Numerical taxonomy of Thermus isolates from hot springs in Portugal. Syst Appl Microbiol 12:310–315 [CrossRef]
    [Google Scholar]
  32. Suzuki K., Collins M. D., Iijima E., Komagata K. 1988; Chemotaxonomic characterization of a radiotolerant bacterium Arthrobacter radiotolerans : description of Rubrobacter radiotolerans gen. nov., comb. nov.. FEMS Microbiol Lett 5233–40 [CrossRef]
    [Google Scholar]
  33. Tenreiro S., Nobre M. F., da Costa M. S. 1995; Thermus silvanus sp. nov. and Thermus chliarophilus sp. nov., two new species related to Thermus ruber but with lower growth temperatures. Int J Syst Bacteriol 45:633–639 [CrossRef]
    [Google Scholar]
  34. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  35. Wait R., Hudson M. J. 1985; The use of picolinyl esters for the characterization of microbial lipids; application to Campylobacter species. Lett Appl Microbiol 1:95–99 [CrossRef]
    [Google Scholar]
  36. White O., Eisen J. A., Heidelberg J. F. 22 other authors 1999; Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286:1571–1577 [CrossRef]
    [Google Scholar]
  37. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
  38. Williams R. A. D., da Costa M. S. 1992; The genus Thermus and related microorganisms. In The Prokaryotes pp  3745–3753 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  39. Yoshinaka T., Yano K., Yanaguchi H. 1973; Isolation of a highly radioresistant bacterium, Arthrobacter radiotolerans nov. sp. Agric Biol Chem 37:2269–2275 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63109-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63109-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error