1887

Abstract

An alkene-degrading, sulfate-reducing bacterium, strain PF2803, was isolated from oil-polluted sediments (Fos Harbour, France). The cells were found to be Gram-negative, non-sporulating, non-motile and to have a slightly curved rod shape. Optimum growth occurred at 1 % (w/v) NaCl, pH 6·8 and 28–30 °C. Strain PF2803 oxidized alkenes (from C to C). The G+C content of the genomic DNA was 57·8 mol% (HPLC). On the basis of 16S rRNA gene sequence analyses, strain PF2803 belongs to the family ‘’ in the class ‘’, with as its closest relative (99·6 % identity). Comparative sequence analyses of the dissimilatory sulfite reductase () gene supported the affiliation of strain PF2803 to the genus . DNA–DNA hybridization with its closest taxon demonstrated 48·4 % similarity. On the basis of the results of physiological and genetic analyses, strain PF2803 is identified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PF2803 (=DSM 16219=ATCC BAA-924).

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2004-09-01
2021-04-17
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