1887

Abstract

Twenty Gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in species-specific PCR tests but that yielded an amplicon in an genus-specific PCR test were isolated from 13 unrelated broiler carcasses. Numerical analysis of the profiles obtained by SDS-PAGE of whole-cell proteins clustered all isolates in a single group distinct from the other species. DNA–DNA hybridization among four representative strains exhibited DNA binding values above 91 %. DNA–DNA hybridization with reference strains of the current four species revealed binding levels below 47 %. The G+C contents ranged between 26·8 and 27·3 mol%. Pairwise comparison of 16S rRNA gene sequences revealed the mean values for similarity to the type strain of (97·5 %), (96·5 %), (96·0 %) and (95·0 %). The levels of similarity to and species were below 88 and 87 %, respectively. The isolates could be distinguished from other species by the following biochemical tests: catalase, oxidase and urease activities; reduction of nitrate; growth at 25 and 37 °C under aerobic conditions; growth on 2–4 % (w/v) NaCl media; and susceptibility to cephalothin. These data demonstrate that the 20 isolates represent a single novel species, for which the name sp. nov. is proposed, with LMG 21996 (=CCUG 48482) as the type strain.

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2005-03-01
2020-09-25
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