1887

Abstract

Twenty Gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in species-specific PCR tests but that yielded an amplicon in an genus-specific PCR test were isolated from 13 unrelated broiler carcasses. Numerical analysis of the profiles obtained by SDS-PAGE of whole-cell proteins clustered all isolates in a single group distinct from the other species. DNA–DNA hybridization among four representative strains exhibited DNA binding values above 91 %. DNA–DNA hybridization with reference strains of the current four species revealed binding levels below 47 %. The G+C contents ranged between 26·8 and 27·3 mol%. Pairwise comparison of 16S rRNA gene sequences revealed the mean values for similarity to the type strain of (97·5 %), (96·5 %), (96·0 %) and (95·0 %). The levels of similarity to and species were below 88 and 87 %, respectively. The isolates could be distinguished from other species by the following biochemical tests: catalase, oxidase and urease activities; reduction of nitrate; growth at 25 and 37 °C under aerobic conditions; growth on 2–4 % (w/v) NaCl media; and susceptibility to cephalothin. These data demonstrate that the 20 isolates represent a single novel species, for which the name sp. nov. is proposed, with LMG 21996 (=CCUG 48482) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63103-0
2005-03-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550713.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63103-0&mimeType=html&fmt=ahah

References

  1. Atabay, H. I., Corry, J. E. L. & On, S. L. W. ( 1998; ). Diversity and prevalence of Arcobacter spp. in broiler chickens. J Appl Microbiol 84, 1007–1016.[CrossRef]
    [Google Scholar]
  2. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of some Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Harmon, K. M. & Wesley, I. V. ( 1996; ). Identification of Arcobacter isolates by PCR. Lett Appl Microbiol 23, 241–244.[CrossRef]
    [Google Scholar]
  5. Houf, K., Tutenel, A., De Zutter, L., Van Hoof, J. & Vandamme, P. ( 2000; ). Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. FEMS Microbiol Lett 193, 89–94.[CrossRef]
    [Google Scholar]
  6. Houf, K., Devriese, L. A., De Zutter, L., Van Hoof, J. & Vandamme, P. ( 2001; ). Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products. Int J Food Microbiol 71, 189–196.[CrossRef]
    [Google Scholar]
  7. Houf, K., De Zutter, L., Van Hoof, J. & Vandamme, P. ( 2002; ). Assessment of the genetic diversity among arcobacters isolated from poultry products by using two PCR-based typing methods. Appl Environ Microbiol 68, 2172–2178.[CrossRef]
    [Google Scholar]
  8. Imberechts, H., Wild, P., Charlier, G., De Greve, H., Lintermans, P. & Pohl, P. ( 1996; ). Characterization of F18 fimbrial genes fedE and fedF involved in adhesion and length of enterotoxemic Escherichia coli strain 107/86. Microb Pathog 21, 183–192.[CrossRef]
    [Google Scholar]
  9. Logan, E. F., Neill, S. D. & Mackie, D. P. ( 1982; ). Mastitis in dairy cows associated with an aerotolerant Campylobacter. Vet Rec 110, 229–230.[CrossRef]
    [Google Scholar]
  10. Mansfield, L. P. & Forsythe, S. J. ( 2000; ). Arcobacter butzleri, A. skirrowii and A. cryaerophilus – potential emerging human pathogens. Rev Med Microbiol 11, 161–170.[CrossRef]
    [Google Scholar]
  11. McClung, C. R., Patriquin, D. G. & Davis, R. E. ( 1983; ). Campylobacter nitrofigilis sp. nov., a nitrogen-fixing bacterium associated with roots of Spartina alterniflora Loisel. Int J Syst Bacteriol 33, 605–612.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. On, S. L. W., Holmes, B. & Sackin, M. J. ( 1996; ). A probability matrix for the identification of campylobacters, helicobacters and allied taxa. J Appl Bacteriol 81, 425–432.
    [Google Scholar]
  14. On, S. L. W., Jensen, T. K., Bille-Hansen, V., Jorsal, S. E. & Vandamme, P. ( 2002; ). Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark. Vet Microbiol 85, 159–167.[CrossRef]
    [Google Scholar]
  15. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  16. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  17. Suarez, D. L., Wesley, I. V. & Larson, D. J. ( 1997; ). Detection of Arcobacter species in gastric samples from swine. Vet Microbiol 4, 325–336.
    [Google Scholar]
  18. Vandamme, P. & De Ley, J. ( 1991; ). Proposal for a new family, Campylobacteraceae. Int J Syst Bacteriol 41, 451–455.[CrossRef]
    [Google Scholar]
  19. Vandamme, P., Vancanneyt, M., Pot, B. & 10 other authors ( 1992; ). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef]
    [Google Scholar]
  20. Wirsen, C. O., Sievert, S. M., Cavanaugh, C. M., Molyneaux, S. J., Ahmad, A., Taylor, L. T., DeLong, E. F. & Taylor, C. D. ( 2002; ). Characterization of an autotrophic sulfide-oxidizing marine Arcobacter sp. that produces filamentous sulfur. Appl Environ Microbiol 68, 316–325.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63103-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63103-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error