1887

Abstract

Morphological, biochemical and molecular genetic studies were carried out on an unknown non-spore-forming, Gram-negative, rod-shaped bacterium which was isolated from dog faeces. The bacterium grew under anaerobic conditions, was asaccharolytic, resistant to 20 % (v/v) bile and was oxidase- and urease-negative. Phylogenetic analysis based on comparative 16S rRNA gene sequencing showed that the unidentified bacterium clustered with , although a sequence divergence of >5 % indicated that the bacterium from dog faeces represented a previously unrecognized subline within the genus. On the basis of the presented findings, a novel species, sp. nov., is described. The type strain of is 5BAC4 (=CCUG 47620=CIP 108024).

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2004-09-01
2020-11-30
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References

  1. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurements of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  2. Engberg J., On S. L., Harrington C. S., Gerner-Smidt P. 2000; Prevalence of Campylobacter , Arcobacter , Helicobacter , and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. J Clin Microbiol 38:286–291
    [Google Scholar]
  3. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  5. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  6. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  7. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4:14
    [Google Scholar]
  8. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computer. Comput Appl Biosci 12:357–358
    [Google Scholar]
  9. Pearson W. R., Lipman D. J. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441 [CrossRef]
    [Google Scholar]
  10. Rasmussen S. W. 2002; SEQtools, a software package for analysis of nucleotide and protein sequences. http://www.seqtools.dk
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  12. Summanen P., Baron E. J., Citron D., Strong C., Wexler H. M., Finegold S. M. 1993 Wadsworth Anaerobic Bacteriology Manual , 5th edn. Belmont, CA: Star Publishing;
    [Google Scholar]
  13. Wexler H. M., Reeves D., Summanen P. H., Molitoris E., McTeague M., Duncan J., Wilson K. H., Finegold S. M. 1996; Sutterella wadsworthensis gen. nov., sp. nov. bile-resistant microaerophilic Campylobacter gracilis -like clinical isolates. Int J Syst Bacteriol 46:252–258 [CrossRef]
    [Google Scholar]
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