1887

Abstract

A total of 128 strains was isolated from more than 23 legume hosts in Korea. Phylogenetic relationships between these Korean isolates and reference strains of the genera , , and were analysed using their 16S rRNA gene and internally transcribed spacer (ITS) region sequences. Among the strains, dendrograms based on both the 16S rRNA gene and ITS region sequences produced two main groups. The ITS tree yielded at least two new clusters that were discernable from the seven previously delineated genospecies. Large discrepancies were revealed between phylogenetic dendrograms based on 16S rRNA gene and ITS region sequences for members of the genus , reflecting their taxonomic heterogeneity. The amalgamation of and former members of was confirmed using the 16S rRNA tree. Phylogenetic analysis of ITS region sequences showed that the clade (group II) and the / clade (group III) could be tentatively recognized as groups that are separable from the core group (group I), which includes . Dendrograms based on the 16S rRNA gene and ITS region sequences of strains were highly conflicting due to the poor taxonomic resolution of the 16S rRNA gene sequences and the low confidence in the ITS dendrogram. Several Korean isolates within the genus are thought to represent novel taxa when considering their relatively low ITS region sequence similarities (<80 %) to the reference strains.

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2005-01-01
2019-10-20
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