1887

Abstract

Forty-eight bacterial strains were isolated at dairy farms from raw milk, the milking apparatus, green fodder or feed concentrate after a heat treatment of 30 min at 100 °C. In this way, spore-forming bacteria with a very high intrinsic heat resistance were selected for. The aerobic spore-forming isolates were subjected to a polyphasic taxonomical study, including repetitive element sequence-based PCR typing, whole-cell protein profiling, 16S rDNA sequence analysis, DNA–DNA hybridizations, DNA base composition, fatty acid analysis, and morphological and biochemical characteristics. A comparison of the REP- and (GTG)-PCR and whole-cell protein SDS-PAGE profiles resulted in three clusters of similar strains. Analysis of the 16S rDNA sequences and DNA–DNA relatedness data showed that these clusters represented three novel species. The highest 16S rDNA similarity to a recognized species found for the three groups was around 94 % with and . Further phenotypic characterization supported the proposal of three novel species in the genus , , and . The respective type strains are R-6540 (=LMG 22081=DSM 16013), R-6514 (=LMG 22079=DSM 16012) and R-7190 (=LMG 22080=DSM 16014); their G+C DNA base contents are 43·7, 44·3 and 41·9 mol%, respectively. Although in variable amounts, a predominance of the branched fatty acids iso-C and anteiso-C was observed in all three novel species.

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2004-07-01
2019-09-18
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Photomicrographs of sporangia and vegetative cells of the type strains of (a) R-6540 (=LMG 22081 =DSM 16013 ), (b) R-6514 (=LMG 22079 =DSM 16012 ) and (c) R-7190 (=LMG 22080 =DSM 16014 ). Bars, 3 µm.

IMAGE

Photomicrographs of sporangia and vegetative cells of the type strains of (a) R-6540 (=LMG 22081 =DSM 16013 ), (b) R-6514 (=LMG 22079 =DSM 16012 ) and (c) R-7190 (=LMG 22080 =DSM 16014 ). Bars, 3 µm.

IMAGE

Photomicrographs of sporangia and vegetative cells of the type strains of (a) R-6540 (=LMG 22081 =DSM 16013 ), (b) R-6514 (=LMG 22079 =DSM 16012 ) and (c) R-7190 (=LMG 22080 =DSM 16014 ). Bars, 3 µm.

IMAGE

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