1887

Abstract

An endospore-forming strain, 20CM, was isolated from Antarctic sediment and identified as a member of the genus on the basis of phenotypic and phylogenetic analyses. The organism stained Gram-variable and was facultatively anaerobic. Strain 20CM was psychrotolerant, growing optimally at 10–15 °C. Like other species, it contained anteiso-C as the major cellular fatty acid. The DNA G+C content was 40·7 mol%. 16S rRNA gene sequence analysis placed strain 20CM within the cluster, with a similarity value of 99·5 % to DSM 2. DNA–DNA hybridization experiments between the Antarctic isolate and DSM 2 revealed a reassociation value of 47 %, indicating that strain 20CM and DSM 2 belong to different species. Based on evaluation of morphological, physiological, chemotaxonomic and phylogenetic analyses, a novel species, sp. nov., is proposed; the type strain is 20CM (=LMG 22078=CECT 5836).

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2004-09-01
2021-04-22
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References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie Van Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Berge O., Guinebretière M.-H., Achouak W., Normand P., Heulin T. 2002; Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52:607–616
    [Google Scholar]
  3. Bozal N., Montes M. J., Tudela E., Jiménez F., Guinea J. 2002; Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52:195–205
    [Google Scholar]
  4. Chung Y. R., Kim C. H., Hwang I., Chun J. 2000; Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int J Syst Evol Microbiol 50:1495–1500 [CrossRef]
    [Google Scholar]
  5. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872, 174AL . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1105–1139 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1993 Manual for the Identification of Medical Bacteria , 3rd edn. Edited and revised by Barrow G. I., Feltham R. K. A. Cambridge: Cambridge University Press;
    [Google Scholar]
  7. Elo S., Suominen I., Kämpfer P., Juhanoja J., Salkinoja-Salonen M., Haahtela K. 2001; Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51:535–545
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  9. Fortina M. G., Pukall R., Schumann P., Mora D., Parini C., Manachini P. L., Stackebrandt E. 2001; Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.. Int J Syst Evol Microbiol 51:447–455
    [Google Scholar]
  10. Gevers D., Huys G., Swings J. 2001; Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
    [Google Scholar]
  11. Heyndrickx M., Vandemeulebroecke K., Scheldeman P. 7 other authors 1995; Paenibacillus (formerly Bacillus ) gordonae (Pichinoty et al . 1986)Ash et al . 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus ) validus (Nakamura 1984) Ash et al . 1994: emended description of P. validus . Int J Syst Bacteriol 45:661–669 [CrossRef]
    [Google Scholar]
  12. Heyndrickx M., Vandemeulebroecke K., Hoste B., Janssen P., Kersters K., De Vos P., Logan N. A., Ali N., Berkeley R. C. W. 1996a; Reclassification of Paenibacillus (formerly Bacillus ) pulvifaciens (Nakamura 1984) Ash et al . 1994, a later subjective synonym ofPaenibacillus (formerly Bacillus ) larvae (White 1906) Ash et al . 1994, as a subspecies of P. larvae , with emended descriptions of P. larvae as P.larvae subsp. larvae and P. larvae subsp. pulvifaciens . Int J Syst Bacteriol 46:270–279 [CrossRef]
    [Google Scholar]
  13. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1996b; A polyphasic reassessment of the genus Paenibacillus , reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb.nov. and of Bacillus peoriae (Montefusco et al . 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae . Int J Syst Bacteriol 46:988–1003 [CrossRef]
    [Google Scholar]
  14. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus . Int J Syst Bacteriol 48:99–106 [CrossRef]
    [Google Scholar]
  15. Hucker G. J., Conn H. J. 1923; Methods of Gram staining . Technical Bulletin of the New York State Agricultural Experimental Station no: 93
    [Google Scholar]
  16. Kanzawa Y., Harada A., Takeuchi M., Yokota A., Harada T. 1995; Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyse resistant curdlan. Int J Syst Bacteriol 45:515–521 [CrossRef]
    [Google Scholar]
  17. Logan N. A., Lebbe L., Hoste B. 7 other authors 2000; Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50:1741–1753
    [Google Scholar]
  18. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodriguez-Diaz M., Heyndrickx M., De Vos P. 2002; Bacillus luciferensis sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 52:1985–1989 [CrossRef]
    [Google Scholar]
  19. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P. 2004a; Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54:373–376 [CrossRef]
    [Google Scholar]
  20. Logan N. A., De Clerck E., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P. 2004b; Paenibacillus cineris sp. nov. and Paenibacillus cookii sp. nov., from Antarctic volcanic soils and a gelatin-processing plant. Int J Syst Evol Microbiol 54: (in press
    [Google Scholar]
  21. Manafi M., Kneifel W. 1990; Rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria. J Appl Bacteriol 69:822–827 [CrossRef]
    [Google Scholar]
  22. Marshall B. J., Ohye D. F. 1966; Bacillus macquariensis n. sp., a psychrotrophic bacterium from sub-Antarctic soil. J Gen Microbiol 44:41–46 [CrossRef]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  24. Nakamura L. K. 1987; Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., two alginate-degrading species. Int J Syst Bacteriol 37:284–286 [CrossRef]
    [Google Scholar]
  25. Nazina T. N., Tourova T. P., Poltaraus A. B. 8 other authors 2001; Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus , Bacillus thermocatenulatus , Bacillus thermoleovorans , Bacillus kaustophilus , Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus , G. thermocatenulatus , G. thermoleovorans , G. kaustophilus , G.thermoglucosidasius and G. thermodenitrificans . Int J Syst Evol Microbiol 51, 433–446
    [Google Scholar]
  26. Niimura Y., Koh E., Yanagida F., Suzuki K.-I., Komagata K., Kozaki M. 1990; Amphibacillus xylanus gen. nov., sp. nov. a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. Int J Syst Bacteriol 40:297–301 [CrossRef]
    [Google Scholar]
  27. Pettersson B., Rippere K. E., Yousten A. A., Priest F. G. 1999; Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov. Int J Syst Bacteriol 49:531–540 [CrossRef]
    [Google Scholar]
  28. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillu s. J Gen Microbiol 134:1847–1882
    [Google Scholar]
  29. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
    [Google Scholar]
  30. Seldin L., Van Elsas J. D., Penido E. G. C. 1984; Bacillus azotofixans sp. nov., a nitrogen-fixing species from Brazilian soils and grass roots. Int J Syst Bacteriol 34:451–456 [CrossRef]
    [Google Scholar]
  31. Seldin L., Penido E. G. C. 1986; Identification of Bacillus azotofixans using API tests. Antonie Van Leeuwenhoek 52:403–409 [CrossRef]
    [Google Scholar]
  32. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946 [CrossRef]
    [Google Scholar]
  33. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997a; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  34. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997b; Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol 47:299–306 [CrossRef]
    [Google Scholar]
  35. Slepecky R. A., Hemphill H. E. 1991; The genus Bacillus – nonmedical. In The Prokaryotes pp  1663–1696 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  36. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with description of Halobacillus litoralis sp.nov. and Halobacillus truperi sp. nov., and transfer of Sporosarcina halophilia to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  37. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  38. van der Maarel M. J. E. C., Veen A., Wijbenga D. J. 2000; Paenibacillus granivorans sp. nov., a new Paenibacillus species which degrades native potato starch granules. Syst Appl Microbiol 23:344–348 [CrossRef]
    [Google Scholar]
  39. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831 [CrossRef]
    [Google Scholar]
  40. Wisotzkey J. D., Jurtshuk P. Jr, Fox G. E., Deinhard G., Poralla K. 1992; Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius , Bacillus acidoterrestris , and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42:263–269 [CrossRef]
    [Google Scholar]
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