1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 12

sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA Free

Abstract

A motile, rod-shaped, pink-pigmented, extremely halophilic archaeon, strain SF3-213, was isolated from a crystallizer pond at the Cargill Solar Salt Plant, Newark, California (USA). Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus , with a close relationship to NRC 34021 (98.6 % similarity), ATCC 33755 (98.3 %) and AS 1.3527 (98.2 %). The polar lipids of strain SF3-213 were CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl-diether. Strain SF3-213 grew in 2.5–5.0 M NaCl. The temperature and pH ranges for growth were 25–42 °C and 6.8–8.5, respectively. Optimal growth occurred at 3.5–4.5 M NaCl, 37 °C and pH 7.3. Mg was required for growth. The DNA G+C content was 69.4 mol%. DNA–DNA hybridization values lower than 70 % were obtained between strain SF3-213 and the closely related species of the genus . Based on the data presented in this study, strain SF3-213 represents a novel species for which the name sp. nov. is proposed; the type strain is SF3-213 (=CECT 7256=DSM 19288=JCM 14715).

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Keyword(s): PG, phosphatidylglycerol , PGP-Me, phosphatidylglycerol phosphate methyl ester , PGS, phosphatidylglycerol sulfate and S-DGD, sulfated diglycosyl-diether
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2008-12-01
2020-03-30
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References

  1. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45, 493–496.
     [Google Scholar]
  2. Benlloch, S., López-López, A., Casamayor, E. O., Øvreås, L., Goddard, V., Daae, F. L., Smerdon, G., Massana, R., Joint, I. & other authors ( 2002; ). Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern. Environ Microbiol 4, 349–360.[CrossRef]
    [Google Scholar]
  3. Brown, E. J. & Braddock, J. F. ( 1990; ). Sheen screen, a miniaturized most-probable-number method for enumeration of oil-degrading microorganisms. Appl Environ Microbiol 56, 3895–3896.
     [Google Scholar]
  4. Castillo, A. M., Gutiérrez, M. C., Kamekura, M., Xue, Y., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A. ( 2006; ). Halorubrum orientale sp. nov., a halophilic archaeon isolated from Lake Ejinor, Inner Mongolia, China. Int J Syst Evol Microbiol 56, 2559–2563.[CrossRef]
    [Google Scholar]
  5. Castillo, A. M., Gutiérrez, M. C., Kamekura, M., Xue, Y., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A. ( 2007; ). Halorubrum ejinorense sp. nov., isolated from Lake Ejinor, Inner Mongolia, China. Int J Syst Evol Microbiol 57, 2538–2542.[CrossRef]
    [Google Scholar]
  6. Cui, H.-L., Tohty, D., Zhou, P.-J. & Liu, S.-J. ( 2006; ). Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China. Int J Syst Evol Microbiol 56, 1631–1634.[CrossRef]
    [Google Scholar]
  7. Cui, H.-L., Lin, Z.-Y., Dong, Y., Zhou, P.-J. & Liu, S.-J. ( 2007; ). Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57, 2204–2206.[CrossRef]
    [Google Scholar]
  8. Fan, H., Xue, Y., Ma, Y., Ventosa, A. & Grant, W. D. ( 2004; ). Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. Int J Syst Evol Microbiol 54, 1213–1216.[CrossRef]
    [Google Scholar]
  9. Feng, J., Zhou, P.-J. & Liu, S.-J. ( 2004; ). Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China. Int J Syst Evol Microbiol 54, 1789–1791.[CrossRef]
    [Google Scholar]
  10. Feng, J., Zhou, P., Zhou, Y.-G., Liu, S.-J. & Warren-Rhodes, K. ( 2005; ). Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Int J Syst Evol Microbiol 55, 149–152.[CrossRef]
    [Google Scholar]
  11. Franzmann, P. D., Stackebrandt, E., Sanderson, K., Volkman, J. K., Cameron, D. E., Stevenson, P. L., McMeekin, T. A. & Burton, H. R. ( 1988; ). Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol 11, 20–27.[CrossRef]
    [Google Scholar]
  12. Grant, W. D. & Ross, H. N. M. ( 1986; ). The ecology and taxonomy of halobacteria. FEMS Microbiol Rev 39, 9–15.[CrossRef]
    [Google Scholar]
  13. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). The order Halobacteriales. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–334. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  14. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Kamekura, M. ( 1998; ). Diversity of extremely halophilic bacteria. Extremophiles 2, 289–295.[CrossRef]
    [Google Scholar]
  16. Kamekura, M. & Dyall-Smith, M. L. ( 1995; ). Taxonomy of the family Halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41, 333–350.[CrossRef]
    [Google Scholar]
  17. Kamekura, M., Dyall-Smith, M. L., Upasani, V., Ventosa, A. & Kates, M. ( 1997; ). Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba, and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47, 853–857.[CrossRef]
    [Google Scholar]
  18. Kharroub, K., Quesada, T., Ferrer, R., Fuentes, S., Aguilera, M., Boulahrouf, A., Ramos-Cormenzana, A. & Monteoliva-Sánchez, M. ( 2006; ). Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria. Int J Syst Evol Microbiol 56, 1583–1588.[CrossRef]
    [Google Scholar]
  19. Litchfield, C. D. & Oren, A. ( 2001; ). Polar lipids and pigments as biomarkers for the study of the microbial community structure of solar salterns. Hydrobiologia 466, 81–89.[CrossRef]
    [Google Scholar]
  20. Litchfield, C. D., Sikaroodi, M. & Gillivet, P. ( 2005; ). The microbial diversity of a solar saltern on San Francisco Bay. In Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya, pp. 59–69. Edited by N. Gunde-Cimerman, A. Oren & A. Plemenitas. Dordrecht, The Netherlands: Springer.
  21. Lizama, C., Monteoliva-Sánchez, M., Suárez-Garciá, A., Roselló-Mora, R., Aguilera, M., Campos, V. & Ramos-Cormenzana, A. ( 2002; ). Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile. Int J Syst Evol Microbiol 52, 149–155.
     [Google Scholar]
  22. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H. ( 1998; ). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef]
    [Google Scholar]
  23. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  24. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  25. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  26. McGenity, T. & Grant, W. D. ( 1995; ). Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18, 237–243.[CrossRef]
    [Google Scholar]
  27. McGenity, T. J. & Grant, W. D. ( 2001; ). Genus VII. Halorubrum. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 320–324. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  28. Mormile, M. R., Romine, M. F., Garcia, M. T., Ventosa, A., Bailey, T. J. & Peyton, B. M. ( 1999; ). Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst Appl Microbiol 22, 551–558.[CrossRef]
    [Google Scholar]
  29. Mwatha, W. E. & Grant, W. D. ( 1993; ). Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int J Syst Bacteriol 43, 401–404.[CrossRef]
    [Google Scholar]
  30. Ochsenreiter, T., Pfeifer, F. & Schleper, C. ( 2002; ). Diversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies. Extremophiles 6, 267–274.[CrossRef]
    [Google Scholar]
  31. Oren, A. ( 1983; ). Halobacterium sodomense sp. nov., a Dead Sea halobacterium with an extremely high magnesium requirement. Int J Syst Bacteriol 33, 381–386.[CrossRef]
    [Google Scholar]
  32. Oren, A. ( 2002a; ). Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 28, 56–63.[CrossRef]
    [Google Scholar]
  33. Oren, A. ( 2002b; ). Molecular ecology of extremely halophilic Archaea and Bacteria. FEMS Microbiol Ecol 39, 1–7.[CrossRef]
    [Google Scholar]
  34. Oren, A. & Ventosa, A. ( 1996; ). A proposal for the transfer of Halorubrobacterium distributum and Halorubrobacterium coriense to the genus Halorubrum as Halorubrum distributum comb. nov. and Halorubrum coriense comb. nov., respectively. Int J Syst Bacteriol 46, 1180 [CrossRef]
    [Google Scholar]
  35. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  36. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists (Society for Applied Bacteriology Technical Series no. 14), 2nd edn, pp. 277–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  37. Soto, C. Y., Cama, M., Gibert, I. & Luquin, M. ( 2000; ). Application of an easy and reliable method for sulfolipid-I detection in the study of its distribution in Mycobacterium tuberculosis strains. FEMS Microbiol Lett 187, 103–107.[CrossRef]
    [Google Scholar]
  38. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  39. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  40. Tomlinson, G. A. & Hochstein, L. I. ( 1976; ). Halobacterium saccharovorum sp. nov., a carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 22, 587–591.[CrossRef]
    [Google Scholar]
  41. Ventosa, A., Gutiérrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
  42. Ventosa, A., Gutiérrez, M. C., Kamekura, M., Zvyagintseva, I. S. & Oren, A. ( 2004; ). Taxonomic study of Halorubrum distributum and proposal of Halorubrum terrestre sp. nov. Int J Syst Evol Microbiol 54, 389–392.[CrossRef]
    [Google Scholar]
  43. Xu, X.-W., Wu, Y.-H., Zhang, H.-b. & Wu, M. ( 2007; ). Halorubrum arcis sp. nov., an extremely halophilic archaeon isolated from a saline lake on the Qinghai–Tibet Plateau, China. Int J Syst Evol Microbiol 57, 1069–1072.[CrossRef]
    [Google Scholar]
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Halorubrum californiense sp. nov., an extreme archaeal halophile isolated from a crystallizer pond at a solar salt plant in California, USA
Int J Syst Evol Microbiol 58, 2710 (2008); https://doi.org/10.1099/ijs.0.2008/002410-0
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