A motile, rod-shaped, pink-pigmented, extremely halophilic archaeon, strain SF3-213, was isolated from a crystallizer pond at the Cargill Solar Salt Plant, Newark, California (USA). Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus , with a close relationship to NRC 34021 (98.6 % similarity), ATCC 33755 (98.3 %) and AS 1.3527 (98.2 %). The polar lipids of strain SF3-213 were CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl-diether. Strain SF3-213 grew in 2.5–5.0 M NaCl. The temperature and pH ranges for growth were 25–42 °C and 6.8–8.5, respectively. Optimal growth occurred at 3.5–4.5 M NaCl, 37 °C and pH 7.3. Mg was required for growth. The DNA G+C content was 69.4 mol%. DNA–DNA hybridization values lower than 70 % were obtained between strain SF3-213 and the closely related species of the genus . Based on the data presented in this study, strain SF3-213 represents a novel species for which the name sp. nov. is proposed; the type strain is SF3-213 (=CECT 7256=DSM 19288=JCM 14715).


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