A Gram-positive, aerobic, motile, coccoid or short rod-shaped bacterium, 5420S-23, was isolated from an air sample collected in the Republic of Korea. According to phylogenetic analysis based on 16S rRNA gene sequences, strain 5420S-23 revealed 97.5, 97.3, 97.3 and 97.2 % similarity, respectively, to DSM 20112, DSM 20118, DSM 20113 and X.bu-b. The peptidoglycan type of strain 5420S-23 was A4, containing -ornithine–-glutamic acid. The cell-wall sugars were galactose, glucose and xylose. The major fatty acids were anteiso-C (49.7 %) and C (20.0 %). The major menaquinone was MK-9(H) and major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 74 mol%. The results of DNA–DNA hybridization with strains of closely related species, in combination with chemotaxonomic and physiological data, demonstrated that isolate 5420S-23 represents a novel species, for which the name sp. nov. is proposed, with strain 5420S-23 (=KACC 20692 =DSM 18649) as the type strain.


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  1. An, D. S., Im, W. T., Yang, H. C., Kang, M. S., Kim, K. K., Jin, L., Kim, M. K. & Lee, S. T.(2005).Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int J Syst Evol Microbiol 55, 1705–1709.[CrossRef] [Google Scholar]
  2. Bagnara, C., Toci, R., Gaudin, C. & Belaich, J. P.(1985). Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 35, 502–507.[CrossRef] [Google Scholar]
  3. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. (editors)(1923).Bergey's Manual of Determinative Bacteriology. Baltimore: Williams & Wilkins.
  4. Collins, M. D. & Pascual, C.(2000). Reclassification of Actinomyces humiferus (Gledhill and Casida) as Cellulomonas humilata nom. corrig., comb. nov. Int J Syst Evol Microbiol 50, 661–663.[CrossRef] [Google Scholar]
  5. Elberson, M. A., Malekzadeh, F., Yazdi, M. T., Kameranpour, N., Noori-Daloii, M. R., Matte, M. H., Shahamat, M., Colwell, R. R. & Sowers, K. R.(2000).Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50, 993–996.[CrossRef] [Google Scholar]
  6. Funke, G., Pascual Ramos, C. & Collins, M. D.(1995). Identification of some clinical strains of CDC coryneform group A-3 and A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. J Clin Microbiol 33, 2091–2097. [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M.(2004).mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  9. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  10. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  11. Rivas, R., Trujillo, M. E., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2004).Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54, 533–536.[CrossRef] [Google Scholar]
  12. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  13. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  14. Seldin, L. & Dubnau, D.(1985). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef] [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Stackebrandt, E. & Kandler, O.(1979). Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology, and proposal of seven neotype strains. Int J Syst Bacteriol 29, 273–282.[CrossRef] [Google Scholar]
  17. Stackebrandt, E. & Schumann, P.(2000). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. Int J Syst Evol Microbiol 50, 1279–1285.[CrossRef] [Google Scholar]
  18. Stackebrandt, E., Breymann, S., Steiner, U., Prauser, H., Weiss, N. & Schumann, P.(2002). Re-evaluation of the status of the genus Oerskovia, reclassification of Promicromonospora enterophila (Jáger et al. 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov. Int J Syst Evol Microbiol 52, 1105–1111.[CrossRef] [Google Scholar]
  19. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  21. Weon, H.-Y., Kim, B.-Y., Yoo, S.-H., Lee, S.-Y., Kwon, S.-W., Go, S.-J. & Stackebrandt, E.(2006).Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56, 1777–1782.[CrossRef] [Google Scholar]
  22. Yi, H., Schumann, P. & Chun, J.(2007).Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 151–156.[CrossRef] [Google Scholar]
  23. Yoon, M.-H., Ten, L. N., Im, W.-T. & Lee, S.-T.(2008).Cellulomonas chitinilytica sp. nov., a novel chitinolytic bacterium isolated from cattle farm compost. Int J Syst Evol Microbiol 58, 1878–1884.[CrossRef] [Google Scholar]

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vol. , part 12, pp. 2925 - 2929

Neighbour-joining tree constructed from a comparative analysis of 16S rRNA gene sequences showing the relationships of strain 5420S-23 with related taxa. [PDF](18 KB)


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