RT Journal Article SR Electronic(1) A1 Gutiérrez, M. C. A1 Castillo, A. M. A1 Kamekura, M. A1 Ventosa, A.YR 2008 T1 Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 12 SP 2880 OP 2884 DO https://doi.org/10.1099/ijs.0.2008/001602-0 PB Microbiology Society, SN 1466-5034, AB A novel extremely halophilic strain, designated XH-65T, isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65T is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum at 3.4 M NaCl, and grows at pH 6.0–9.0, with optimum growth at pH 7.5. Strain XH-65T grows at 25–50 °C, with optimal growth at 37 °C. Magnesium is not required for growth. On the basis of 16S rRNA gene sequence analysis, strain XH-65T was shown to belong to the genus Haloterrigena and was related to Haloterrigena turkmenica VKM B-1734T (98.1 % sequence similarity), Haloterrigena saccharevitans AB14T (96.9 %), Haloterrigena thermotolerans PR5T (96.3 %), Haloterrigena limicola AX-7T (95.8 %) and Haloterrigena hispanica FP1T (95.7 %). DNA–DNA hybridization revealed 37 % relatedness between strain XH-65T and Htg. turkmenica VKM B-1734T. The polar lipid composition revealed the presence of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S2-DGD). The results of the DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain XH-65T from the six Haloterrigena species with validly published names. Therefore, strain XH-65T represents a novel species, for which the name Haloterrigena salina sp. nov. is proposed, with the type strain XH-65T (=CGMCC 1.6203T =JCM 13891T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.2008/001602-0