1887

Abstract

A pale-pink strain (CCUG 53370) from water was investigated by a polyphasic taxonomic approach. The cells stained Gram-positive and were rod-shaped and non-spore-forming. Analyses using the 16S rRNA gene sequence of the isolate showed that the organism belongs to the genus , with the highest sequence similarities to the type strains of (94.4 %), (94.3 %) and (94.3 %). Chemotaxonomic data revealed that CCUG 53370 contains exclusively menaquinone MK-8 as the respiratory quinone and a complex polar lipid profile consisting of different unidentified glycolipids and polar lipids, two unknown phospholipids and three unknown phosphoglycolipids. As in other deinococci, one of these phosphoglycolipids was predominant in the profile, and it was identified in as 2′--(1,2-diacyl--glycero-3-phospho)-3′--(-galactosyl)---glyceroyl alkylamine. Predominant fatty acids were C 7, C 8 and C 9. Biochemical and chemotaxonomic properties demonstrate that strain CCUG 53370 represents a novel species, for which the name sp. nov. is proposed. The type strain is CCUG 53370 (=CCM 7524).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/001206-0
2008-12-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2803.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/001206-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J.(1996). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef] [Google Scholar]
  2. Anderson, R. & Hansen, K.(1985). Structure of a novel phosphoglycolipid from Deinococcus radiodurans. J Biol Chem 260, 12219–12223. [Google Scholar]
  3. Asker, D., Awad, T. S., Beppu, T. & Ueda, K.(2009).Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. Int J Syst Evol Microbiol 59 (in press). [Google Scholar]
  4. Busse, H.-J. & Auling, G.(1988). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef] [Google Scholar]
  5. Callegan, R. P., Nobre, M. F., McTernan, P. M., Battista, J. R., Navarro-González, R., McKay, C. P., da Costa, M. S. & Rainey, F. A.(2008). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. Int J Syst Evol Microbiol 58, 1252–1258.[CrossRef] [Google Scholar]
  6. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  7. de Groot, A., Chapon, V., Servant, P., Christen, R., Fischer-Le Saux, M., Sommer, S. & Heulin, T.(2005).Deinococcus deserti sp. nov., a gamma-radiation tolerant bacterium isolated from the Sahara desert. Int J Syst Evol Microbiol 55, 2441–2446.[CrossRef] [Google Scholar]
  8. Embley, T. M., O'Donnell, A. G., Watt, R. & Rostron, J.(1987). Lipid and cell wall amino acid composition in the classification of members of the genus Deinococcus. Syst Appl Microbiol 10, 20–27.[CrossRef] [Google Scholar]
  9. Ferreira, A. C., Nobre, F. M., Rainey, F. A., Silva, M. T., Wait, R., Burghardt, J., Chung, A. P. & Da Costa, M. S.(1997).Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47, 939–947.[CrossRef] [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) (1994). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Hamana, K.(1994). Polyamine distribution patterns in aerobic Gram-positive cocci and some radio-resistant bacteria. J Gen Appl Microbiol 40, 181–195.[CrossRef] [Google Scholar]
  12. Hirsch, P., Gallikowski, C. A., Siebert, J., Peissl, K., Kroppenstedt, R. M., Schumann, P., Stackebrandt, E. & Anderson, R.(2004).Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27, 636–645.[CrossRef] [Google Scholar]
  13. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  14. Kämpfer, P., Steiof, M. & Dott, W.(1991). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef] [Google Scholar]
  15. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J.(2003).Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef] [Google Scholar]
  16. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  17. Lai, W.-A., Kämpfer, P., Arun, A. B., Shen, F.-T., Huber, B., Rekha, P. D. & Young, C.-C.(2006).Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int J Syst Evol Microbiol 56, 787–791.[CrossRef] [Google Scholar]
  18. Rainey, F. A., Nobre, M. F., Schumann, P., Stackebrandt, E. & Da Costa, M. S.(1997). Phylogenetic diversity of Deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47, 510–514.[CrossRef] [Google Scholar]
  19. Rainey, F. A., Ray, K., Ferreira, M., Gatz, B. Z., Nobre, M. F., Bagaley, D., Rash, B. A., Park, M.-J., Earl, A. M. & other authors(2005). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus from a single soil sample. Appl Environ Microbiol 71, 5225–5235.[CrossRef] [Google Scholar]
  20. Rainey, F. A., Ferreira, M., Nobre, M. F., Ray, K., Bagaley, D., Earl, A. M., Battista, J. R., Gómez-Silva, B., McKay, C. P. & da Costa, M. S.(2007).Deinococcus peraridilitoris sp. nov., isolated from a coastal desert. Int J Syst Evol Microbiol 57, 1408–1412.[CrossRef] [Google Scholar]
  21. Shashidhar, R. & Bandekar, J. R.(2006).Deinococcus mumbaiensis sp. nov., a radiation-resistant pleomorphic bacterium isolated from Mumbai, India. FEMS Microbiol Lett 254, 275–280.[CrossRef] [Google Scholar]
  22. Stolz, A., Busse, H.-J. & Kämpfer, P.(2007).Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef] [Google Scholar]
  23. Suresh, K., Reddy, G. S. N., Sengupta, S. & Shivaji, S.(2004).Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54, 457–461.[CrossRef] [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  25. Tindall, B. J.(1990a). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  26. Tindall, B. J.(1990b). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef] [Google Scholar]
  27. Zhang, Y. Q., Sun, C. H., Li, W. J., Yu, L. Y., Zhou, J. Q., Zhang, Y. Q., Yu, L. H. & Jiang, C.(2007).Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. Int J Syst Evol Microbiol 57, 370–375.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.2008/001206-0
Loading
/content/journal/ijsem/10.1099/ijs.0.2008/001206-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2803 - 2806

Long-chain fatty acid compositions of selected species. [PDF](133 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error