Two actinobacterial strains, YIM 65001 and YIM 65002, were isolated from surface-sterilized plant tissues collected from Yunnan Province, south-west China, and their taxonomic positions were determined by using a polyphasic approach. The DNA G+C contents of strains YIM 65001 and YIM 65002 were 71.9 and 72.6 mol%, respectively. The two strains had chemotaxonomic markers that were consistent with their classification in the genus . Phylogenetic analysis based on almost-complete 16S rRNA gene sequences indicated that strain YIM 65001 was related most closely to DSM 43672 and that strain YIM 65002 was related most closely to CBS 107.95. Levels of 16S rRNA gene sequence similarity between strains YIM 65001 and YIM 65002 and the type strains of other recognized members of the genus were 95.8–99.8 %. DNA–DNA hybridization experiments confirmed the separate genomic status of strains YIM 65001 and YIM 65002. Strains YIM 65001 and YIM 65002 showed significant phenotypic differences between each other and their closest recognized neighbours. On the basis of their phenotypic and phylogenetic distinctiveness, the two novel isolates were identified as representing two novel species of the genus , for which the names sp. nov. (type strain YIM 65001=CCTCC AA 207015=DSM 45139) and sp. nov. (type strain YIM 65002=CCTCC AA 207016=DSM 45140) are proposed.


Article metrics loading...

Loading full text...

Full text loading...



  1. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. Colquhoun, J. A., Heald, S. C., Li, L., Tamaoka, J., Kato, C., Horikoshi, K. & Bull, A. T.(1998). Taxonomy and biotransformation activities of some deep-sea actinomycetes. Extremophiles 2, 269–277.[CrossRef] [Google Scholar]
  4. Coombs, J. T. & Franco, C. M. M.(2003). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef] [Google Scholar]
  5. Duckworth, A. W., Grant, S., Grant, W. D., Jones, B. E. & Meijer, D.(1998).Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2, 359–366.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Goodfellow, M.(1986). Genus Rhodococcus Zopf 1891, 28AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1472–1481. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  8. Harrison, F. C.(1929). The discoloration of halibut. Can J Res 1, 214–239.[CrossRef] [Google Scholar]
  9. He, L., Li, W., Huang, Y., Wang, L. M., Liu, Z. H., Lanoot, B. J., Vancanneyt, M. & Swings, J.(2005).Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef] [Google Scholar]
  10. Jones, A. L., Koerner, R. J., Natarajan, S., Perry, J. D. & Goodfellow, M.(2008).Dietzia papillomatosis sp. nov., a novel actinomycete isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis. Int J Syst Evol Microbiol 58, 68–72.[CrossRef] [Google Scholar]
  11. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  12. Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178, 703–704. [Google Scholar]
  13. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  14. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  15. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  16. Mayilraj, S., Suresh, K., Kroppenstedt, R. M. & Saini, H. S.(2006).Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 56, 1667–1671.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 17–22. [Google Scholar]
  19. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef] [Google Scholar]
  20. Nesterenko, O. A., Nogina, T. M., Kasumova, S. A., Kvasnikov, E. I. & Batrakov, S. G.(1982).Rhodococcus luteus nom. nov. and Rhodococcus maris nom nov. Int J Syst Bacteriol 32, 1–14.[CrossRef] [Google Scholar]
  21. Rainey, F. A., Klatte, S., Kroppenstedt, R. M. & Stackebrandt, E.(1995).Dietzia, a new genus including Dietzia maris comb. nov., formerly Rhodococcus maris. Int J Syst Bacteriol 45, 32–36.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  25. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  26. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  29. Xu, P., Li, W. J., Tang, S. K., Zhang, Y. Q., Chen, G. Z., Chen, H. H., Xu, L. H. & Jiang, C. L.(2005).Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55, 1149–1153.[CrossRef] [Google Scholar]
  30. Yassin, A. F., Hupfer, H. & Schaal, K. P.(2006).Dietzia cinnamea sp. nov., a novel species isolated from a perianal swab of a patient with a bone marrow transplant. Int J Syst Evol Microbiol 56, 641–645.[CrossRef] [Google Scholar]
  31. Yumoto, I., Nakamura, A., Iwata, H., Kojima, K., Kusumoto, K., Nodasaka, Y. & Matsuyama, H.(2002).Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons. Int J Syst Evol Microbiol 52, 85–90. [Google Scholar]

Data & Media loading...


Two-dimensional thin-layer chromatograms of phospholipids of strains YIM 65001 ( sp. nov.) and YIM 65002 ( sp. nov.). [PDF](57 KB)


Fatty acid profiles of strains YIM 65001 ( sp. nov.) and YIM 65002 ( sp. nov.) and the type strains of closely related species. [PDF](47 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error