1887

International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 12

sp. nov., a ferrous-oxidizing bacterium from solfataric soil Free

Abstract

A novel mesophilic and acidophilic Gram-positive bacterium, designated strain TC-34, was isolated from solfataric soil. Strain TC-34 grew aerobically at 17–40 °C and pH 2.0–6.0, and optimally at 28 °C and pH 3.0. Analysis based on 16S rRNA gene sequences showed that strain TC-34 was phylogenetically related to members of the genus , with the highest similarity (94.8 %) to . Strain TC-34 showed a range of phenotypic characteristics that differentiated it from recognized species, including growth temperature, assimilation of carbon sources and production of acids from a range of compounds. Strain TC-34 was able to oxidize ferrous iron and its growth was correlated with the oxidation of Fe in culture medium. -Alicyclic fatty acids were not detected. On the basis of these results, it was concluded that strain TC-34 represents a novel species of the genus , for which the name (type strain TC-34=JCM 15090=CGMCC 1.6357) is proposed.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/000562-0
2008-12-01
2021-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2898.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/000562-0&mimeType=html&fmt=ahah

References

  1. Albuquerque, L., Rainey, F. A., Chung, A. P., Sunna, A., Nobre, M. F., Grote, R., Antranikian, G. & De Costa, M. S. ( 2000; ). Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. Int J Syst Evol Microbiol 50, 451–457.[CrossRef]
    [Google Scholar]
  2. Deinhard, G., Blanz, P., Poralla, K. & Alton, E. ( 1987a; ). Bacillus acidoterrestris sp. nov., a new thermotolerant acidophile isolated from different soils. Syst Appl Microbiol 10, 47–53.[CrossRef]
    [Google Scholar]
  3. Deinhard, G., Saar, J., Krischke, W. & Poralla, K. ( 1987b; ). Bacillus cycloheptanicus sp. nov., a new thermoacidophile containing ω-cycloheptane fatty acids. Syst Appl Microbiol 10, 68–73.[CrossRef]
    [Google Scholar]
  4. Dufresne, S., Bousquet, J., Boissinot, M. & Guay, R. ( 1996; ). Sulfobacillus disulfidooxidans sp. nov., a new acidophilic, disulfide oxidizing, Gram-positive, spore-forming bacterium. Int J Syst Bacteriol 46, 1056–1064.[CrossRef]
    [Google Scholar]
  5. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  6. Goto, K., Mochida, K., Asahara, M., Suzuki, M. & Yokota, A. ( 2002; ). Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus. J Gen Appl Microbiol 48, 243–250.[CrossRef]
    [Google Scholar]
  7. Goto, K., Mochida, K., Asahara, M., Suzuki, M., Kasai, H. & Yokota, K. ( 2003; ). Alicyclobacillus pomorum sp. nov., a novel thermoacidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol 53, 1537–1544.[CrossRef]
    [Google Scholar]
  8. Goto, K., Mochida, K., Kato, Y., Asahara, M., Fujita, R., An, S.-Y., Kasai, H. & Yokota, A. ( 2007; ). Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov. Int J Syst Evol Microbiol 57, 1276–1285.[CrossRef]
    [Google Scholar]
  9. Hippchen, B., Roll, A. & Poralla, K. ( 1981; ). Occurrence in soil of thermo-acidophilic bacilli possessing ω-cyclohexane fatty acids and hopanoids. Arch Microbiol 129, 53–55.[CrossRef]
    [Google Scholar]
  10. Hiraishi, A., Inagaki, K., Tanimoto, Y., Iwasaki, M., Kishimoto, N. & Tanaka, H. ( 1997; ). Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot spring in Japan. J Gen Appl Microbiol 43, 295–304.[CrossRef]
    [Google Scholar]
  11. Imperio, T., Viti, C. & Marri, L. ( 2008; ). Alicyclobacillus pohliae sp. nov., a thermophilic, endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica). Int J Syst Evol Microbiol 58, 221–225.[CrossRef]
    [Google Scholar]
  12. Karavaiko, G. I., Bogdanova, T. I., Tourova, T. P., Kondrat'eva, T. F., Tsaplina, I. A., Egorova, M. A., Krasil'nikova, E. N. & Zakharchuk, L. M. ( 2005; ). Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus. Int J Syst Evol Microbiol 55, 941–947.[CrossRef]
    [Google Scholar]
  13. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  14. Kovalenko, E. V. & Malakhova, P. T. ( 1983; ). The spore-forming ironoxidizing bacterium Sulfobacillus thermosulfidooxidans. Microbiology (English translation of Mikrobiologiia) 52, 962–966.
     [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  16. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  17. Nicolaus, B., Improta, R., Manca, M. C., Lama, L., Esposito, E. & Gambacorta, A. ( 1998; ). Alicyclobacilli from an unexplored geothermal soil in Antarctica: Mount Rittmann. Polar Biol 19, 133–141.[CrossRef]
    [Google Scholar]
  18. Norris, P. R., Clark, D. A. & Owen, J. P. ( 1996; ). Characteristics of Sulfobacillus acidophilus sp. nov. and other moderately thermophilic mineral-sulphide-oxidizing bacteria. Microbiology 142, 775–783.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
     [Google Scholar]
  20. Simbahan, J., Drijber, R. & Blum, P. ( 2004; ). Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Spring, California, USA. Int J Syst Evol Microbiol 54, 1703–1707.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Tsuruoka, N., Isono, Y., Shida, O., Hemmi, H., Nakayama, T. & Nishio, T. ( 2003; ). Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. Int J Syst Evol Microbiol 53, 1081–1084.[CrossRef]
    [Google Scholar]
  23. Wisotzkey, J. D., Jurtshuk, P. Jr, Fox, G. E., Deinhard, G. & Poralla, K. ( 1992; ). Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42, 263–269.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/000562-0
Loading
Alicyclobacillus ferrooxydans sp. nov., a ferrous-oxidizing bacterium from solfataric soil
Int J Syst Evol Microbiol 58, 2898 (2008); https://doi.org/10.1099/ijs.0.2008/000562-0
/content/journal/ijsem/10.1099/ijs.0.2008/000562-0
/content/journal/ijsem/10.1099/ijs.0.2008/000562-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2898 - 2903

Cellular fatty acid compositions of type/reference stains of sp. nov. and releated species. [PDF](23 KB)

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error