1887

Abstract

A Gram-positive, halophilic bacterium was isolated from a sediment sample from Ai-Ding salt lake in China. The isolate, designated strain 17-5, grew at salinities of 8–33 % (w/v) NaCl (optimally at 12 %, w/v). The genomic DNA G+C content of strain 17-5 was 48.1 mol%. The predominant isoprenoid quinone was MK-7(H) and the cell-wall peptidoglycan contained -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 17-5 was a member of the genus , being most closely related to JCM 14087 (96.0 % sequence similarity) and DSM 16461 (95.6 %). The levels of 16S rRNA gene sequence similarity with respect to other species were less than 91.7 %. Comparative analysis of the 16S rRNA gene sequence data, chemotaxonomy and phenotypic features of the novel isolate and related species of indicated that strain 17-5 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 17-5 (=CGMCC 1.3227=DSM 18341).

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2008-12-01
2020-01-24
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References

  1. Albert, R. A., Archambault, J., Rosselló-Mora, R., Tindall, B. J. & Matheny, M. ( 2005; ). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55, 2125–2130.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Cui, H. L., Tohty, D., Zhou, P. J. & Liu, S. J. ( 2006a; ). Halorubrum lipolyticum sp. nov. and Halorubrum aidingense sp. nov., isolated from two salt lakes in Xin-Jiang, China. Int J Syst Evol Microbiol 56, 1631–1634.[CrossRef]
    [Google Scholar]
  4. Cui, H. L., Yang, Y., Dilbr, T., Zhou, P. J. & Liu, S. J. ( 2006b; ). Biodiversity of halophilic archaea isolated from two salt lakes in Xin-Jiang region of China. Wei Sheng Wu Xue Bao 46, 171–176 (in Chinese).
    [Google Scholar]
  5. Duckworth, A. W., Grant, W. D., Jones, B. E. & van Steenbergen, R. ( 1996; ). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef]
    [Google Scholar]
  6. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Phillips, G. B. (editors) ( 1981; ). Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  7. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  8. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  9. Heyrman, J., Vanparys, B., Logan, N. A., Balcaen, A., Rodriguez-Diaz, M., Felske, A. & De Vos, P. ( 2004; ). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus botaviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54, 47–57.[CrossRef]
    [Google Scholar]
  10. Heyrman, J., Rodriguez-Diaz, M., Devos, J., Felske, A., Logan, N. A. & De Vos, P. ( 2005; ). Bacillus novalis sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 55, 111–117.[CrossRef]
    [Google Scholar]
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Kates, M. ( 1986; ). Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier.
  13. Kodaka, H., Armfield, A. Y., Lombard, G. L. & Dowell, V. R., Jr ( 1982; ). Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16, 948–952.
    [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  15. Lim, J.-M., Jeon, C. O. & Kim, C.-J. ( 2006a; ). Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 56, 2903–2908.[CrossRef]
    [Google Scholar]
  16. Lim, J.-M., Jeon, C. O., Lee, S.-M., Lee, J.-C., Xu, L.-H., Jiang, C.-L. & Kim, C.-J. ( 2006b; ). Bacillus salarius sp. nov., a halophilic spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56, 373–377.[CrossRef]
    [Google Scholar]
  17. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  18. Priest, F. G., Goodfellow, M. & Todd, C. ( 1988; ). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882.
    [Google Scholar]
  19. Ren, P.-G. & Zhou, P.-J. ( 2005a; ). Tenuibacillus multivorans gen. nov., sp. nov., a novel moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  20. Ren, P.-G. & Zhou, P.-J. ( 2005b; ). Salinibacillus aidingensis gen. nov., sp. nov. and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 949–953.[CrossRef]
    [Google Scholar]
  21. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  23. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  24. Stackebrandt, E., Fredericksen, W., Garrity, G. M., Grimont, P. A., Kämpfer, P., Maiden, M. C., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  26. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  27. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  28. Wang, Q. F., Li, W., Liu, Y. L., Cao, H. H., Li, Z. & Guo, G. Q. ( 2007; ). Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China. Int J Syst Evol Microbiol 57, 1143–1147.[CrossRef]
    [Google Scholar]
  29. Wieser, M., Worliczek, H., Kämpfer, P. & Busse, H.-J. ( 2005; ). Bacillus herbersteinensis sp. nov. Int J Syst Evol Microbiol 55, 2119–2123.[CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 2828 - 2832

Photomicrographs of cells of strain 17-5 ( sp. nov.). [PDF](212 KB)



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