A marine bacterial strain, designated FR1330, was isolated from a seawater sample collected near Ganghwa Island, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FR1330 belonged to the and was related to the genus ; its closest neighbours were the type strains of (97.9 % sequence similarity) and (95.0 %). DNA–DNA relatedness values for strain FR1330 with CC-YY255 and KACC 11391 were 33 and 10 %, respectively. Cells of strain FR1330 were Gram-negative, aerobic, rod-shaped and oxidase- and catalase-positive. The predominant respiratory lipoquinone was ubiquinone-8. The major fatty acids were branched-chain saturated iso-C (26.2 %) and unsaturated iso-C 9 (26.0 %). The DNA G+C content was 67.6 mol%. On the basis of several phenotypic characteristics, strain FR1330 could be differentiated from and . The data obtained from the polyphasic study demonstrated clearly that strain FR1330 represents a novel species of the genus . The name sp. nov. is proposed, with strain FR1330 (=KCTC 12327=JCM 12488=IMSNU 60306) as the type strain.


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  1. Baik, K. S., Park, Y.-D., Seong, C. N., Kim, E. M., Bae, K. S. & Chun, J.(2006).Glaciecola nitratireducens sp. nov., isolated from sea water. Int J Syst Evol Microbiol 56, 2185–2188.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  4. Busse, H.-J., Kämpfer, P., Moore, E. R. B., Nuutinen, J., Tsitko, I. V., Denner, E. B. M., Vauterin, L., Valens, M., Rosselló-Mora, R. & Salkinoja-Salonen, M. S.(2002).Thermomonas haemolytica gen. nov., sp. nov., a γ-proteobacterium from kaolin slurry. Int J Syst Evol Microbiol 52, 473–483. [Google Scholar]
  5. Chun, J. & Goodfellow, M.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef] [Google Scholar]
  6. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J.(2000).Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913. [Google Scholar]
  7. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  8. CLSI(2003).Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th edn. Approved Standard M2–A8. Wayne, PA: Clinical Laboratory Standards Institute.
  9. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  10. Collins, M. D. & Jones, D.(1981). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45, 316–354. [Google Scholar]
  11. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  12. Finkmann, W., Altendorf, K., Stackebrandt, E. & Lipski, A.(2000). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50, 273–282.[CrossRef] [Google Scholar]
  13. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef] [Google Scholar]
  14. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703 [Google Scholar]
  16. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  17. MIDI(1999).Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Seldin, L. & Dubnau, D.(1985). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef] [Google Scholar]
  20. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  21. Swofford, D. L.(1998). Phylogenetic analysis using parsimony (paup). Version 4. Sunderland, MA: Sinauer Associates.
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  23. Yamaguchi, S. & Yokoe, M.(2000). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66, 3337–3343.[CrossRef] [Google Scholar]
  24. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K.(1992). Distribution of quinone systems in microorganisms: gram-negative Eubacteria. Bull Jpn Fed Cult Coll 8, 136–171. [Google Scholar]
  25. Young, C.-C., Kämpfer, P., Chen, W.-M., Yen, W.-S., Arun, A. B., Lai, W.-A., Shen, F.-T., Rekha, P. D., Lin, K.-Y. & Chou, J.-H.(2007).Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 57, 741–744.[CrossRef] [Google Scholar]

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Fitch-Margoliash tree based on 16S rRNA gene sequences. [PDF](20 KB)


Transmission electron micrograph of cells of strain FR1330 ( sp. nov.). [PDF](238 KB)

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