During the characterization of symbiotic bacteria of Hungarian entomopathogenic nematode isolates, a number of bacteria (including strain 3107) isolated from and showed only moderate 16S rRNA gene sequence similarity to the type strains of all described species and subspecies. On the basis of the 16S rRNA gene sequence, the phylogenetic relationships of these isolates were uncertain, because of the low bootstrap values. Using sequences for phylogenetic analysis, these isolates were shown to be part of the species , with clear separation from both Palaearctic and American strains (phylogenetic distances are 6.9 and 7.9 %, respectively). Physiological properties and carbon-source utilization profiles supported the phylogenetic position of these strains; therefore, a novel subspecies, subsp. subsp. nov. is proposed, with the type strain 3107 (=DSM 19724 =NCAIM B 02271).


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Strain details and GenBank accession numbers of sequences used in the phylogenetic analysis. [PDF](52 KB)


Phylogenetic relationships between the genus based on the analysis of 16S rRNA gene sequence. Evolutionary history was inferred using the neighbour-joining method. [PDF](490 KB)


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