1887

Abstract

A Gram-reaction-positive, catalase-negative, facultatively anaerobic, rod-shaped lactic acid bacterium, designated strain S215, was isolated from fermented soybean meal. The organism produced -lactic acid from glucose without gas formation. 16S rRNA gene sequencing results showed that strain S215 had 98.74–99.60 % sequence similarity to the type strains of three species of the genus ( BCRC 14043, BCRC 80278 and JCM 15951). A comparison of two housekeeping genes, and , revealed that strain S215 was well separated from the reference strains of species of the genus . DNA–DNA hybridization results indicated that strain S215 had DNA related to the three type strains of species of the genus (33–66 % relatedness). The DNA G+C content of strain S215 was 36.2 mol%. The cell walls contained peptidoglycan of the --diaminopimelic acid type and the major fatty acids were Cω9, C and C cyclo ω10/Cω6. Phenotypic and genotypic features demonstrated that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S215 ( = NBRC 109509 = BCRC 80582).

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2015-01-01
2019-11-13
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