1887

Abstract

A Gram-reaction-positive, catalase-negative, facultatively anaerobic, rod-shaped lactic acid bacterium, designated strain S215, was isolated from fermented soybean meal. The organism produced -lactic acid from glucose without gas formation. 16S rRNA gene sequencing results showed that strain S215 had 98.74–99.60 % sequence similarity to the type strains of three species of the genus ( BCRC 14043, BCRC 80278 and JCM 15951). A comparison of two housekeeping genes, and , revealed that strain S215 was well separated from the reference strains of species of the genus . DNA–DNA hybridization results indicated that strain S215 had DNA related to the three type strains of species of the genus (33–66 % relatedness). The DNA G+C content of strain S215 was 36.2 mol%. The cell walls contained peptidoglycan of the --diaminopimelic acid type and the major fatty acids were Cω9, C and C cyclo ω10/Cω6. Phenotypic and genotypic features demonstrated that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S215 ( = NBRC 109509 = BCRC 80582).

Funding
This study was supported by the:
  • National Science Council of Taiwan (Award NSC 101-2313-B-005-009-MY3)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.070938-0
2015-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/1/101.html?itemId=/content/journal/ijsem/10.1099/ijs.0.070938-0&mimeType=html&fmt=ahah

References

  1. Batal A. B., Parsons C. M. ( 2003 ). Utilization of different soy products as affected by age in chicks. . Poult Sci 82, 454462. [View Article] [PubMed]
    [Google Scholar]
  2. Chao S. H., Kudo Y., Tsai Y. C., Watanabe K. ( 2012 ). Lactobacillus futsaii sp. nov., isolated from fu-tsai and suan-tsai, traditional Taiwanese fermented mustard products. . Int J Syst Evol Microbiol 62, 489494. [View Article] [PubMed]
    [Google Scholar]
  3. Chen Y. S., Yanagida F., Hsu J. S. ( 2006 ). Isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in Taiwan. . J Appl Microbiol 101, 125130. [View Article] [PubMed]
    [Google Scholar]
  4. Chen C. C., Shih Y. C., Chiou P. W. S., Yu B. ( 2010a ). Evaluating nutrition quality of single stage- and two stage-fermented soybean meal. . Asian Austral J Anim Sci 23, 598606. [View Article]
    [Google Scholar]
  5. Chen Y. S., Miyashita M., Suzuki K. I., Sato H., Hsu J. S., Yanagida F. ( 2010b ). Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia). . Int J Syst Evol Microbiol 60, 19141917. [View Article] [PubMed]
    [Google Scholar]
  6. Chen C. C., Lee T. T., Hsu C. B., Huang C. W., Yu B. ( 2011 ). Associations of allergenic soybean proteins with piglet skin allergic reaction and application of polyclonal antibodies. . Anim Prod Sci 51, 10081014. [View Article]
    [Google Scholar]
  7. Chen Y. S., Chang C. H., Pan S. F., Wang L. T., Chang Y. C., Wu H. C., Yanagida F. ( 2013 ). Lactococcus taiwanensis sp. nov., a lactic acid bacterium isolated from fresh cummingcordia. . Int J Syst Evol Microbiol 63, 24052409. [View Article] [PubMed]
    [Google Scholar]
  8. De Bruyne K., Camu N., De Vuyst L., Vandamme P. ( 2009 ). Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations. . Int J Syst Evol Microbiol 59, 712. [View Article] [PubMed]
    [Google Scholar]
  9. Dunsford B. R., Knabe D. A., Haensly W. E. ( 1989 ). Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig. . J Anim Sci 67, 18551863.[PubMed]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  11. Farrow J. A. E., Kruze J., Phillips B. A., Bramley A. J., Collins M. D. ( 1984 ). Taxonomic studies on Streptococcus bovis and Streptococcus equinus: description of Streptococcus alactolyticus sp. nov. and Streptococcus saccharolyticus sp. nov.. Syst Appl Microbiol 5, 467482. [View Article]
    [Google Scholar]
  12. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  13. Feng J., Liu X., Xu Z. R., Lu Y. P., Liu Y. Y. ( 2007 ). Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets. . Dig Dis Sci 52, 18451850. [View Article] [PubMed]
    [Google Scholar]
  14. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  15. Fitch W. M. ( 1977 ). On the problem of discovering the most parsimonious tree. . Am Nat 111, 223257. [View Article]
    [Google Scholar]
  16. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. ( 1998 ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44, 11481153. [View Article]
    [Google Scholar]
  17. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  18. Kishida T., Ataki H., Takebe M., Ebihara K. ( 2000 ). Soybean meal fermented by Aspergillus awamori increases the cytochrome P-450 content of the liver microsomes of mice. . J Agric Food Chem 48, 13671372. [View Article] [PubMed]
    [Google Scholar]
  19. Kitahara M., Sakamoto M., Benno Y. ( 2010 ). Lactobacillus similis sp. nov., isolated from fermented cane molasses. . Int J Syst Evol Microbiol 60, 187190. [View Article] [PubMed]
    [Google Scholar]
  20. Li Z., Ding S., Li Z., Tan T. ( 2006 ). l-Lactic acid production by Lactobacillus casei fermentation with corn steep liquor-supplemented acid-hydrolysate of soybean meal. . Biotechnol J 1, 14531458. [View Article] [PubMed]
    [Google Scholar]
  21. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005 ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  22. Reuter G. ( 1983 ). Lactobacillus alimentarius sp. nov., nom rev. and Lactobacillus farciminis sp. nov., nom. rev.. Syst Appl Microbiol 4, 277279. [View Article] [PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  24. Scheirlinck I., Van der Meulen R., Van Schoor A., Huys G., Vandamme P., De Vuyst L., Vancanneyt M. ( 2007 ). Lactobacillus crustorum sp. nov., isolated from two traditional Belgian wheat sourdoughs. . Int J Syst Evol Microbiol 57, 14611467. [View Article] [PubMed]
    [Google Scholar]
  25. Stackebrandt E. G., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  26. Švec P., Vancanneyt M., Seman M., Snauwaert C., Lefebvre K., Sedláček I., Swings J. ( 2005 ). Evaluation of (GTG)5-PCR for identification of Enterococcus spp.. FEMS Microbiol Lett 247, 5963. [View Article] [PubMed]
    [Google Scholar]
  27. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  30. Tohno M., Kitahara M., Irisawa T., Inoue H., Uegaki R., Ohkuma M., Tajima K. ( 2013 ). Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol 63, 29572962. [View Article] [PubMed]
    [Google Scholar]
  31. Versalovic J., Schneider M., De Bruijn F. J., Lupski J. R. ( 1994 ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. . Methods Mol Cell Biol 5, 2540.
    [Google Scholar]
  32. Wang L. T., Lee F. L., Tai C. J., Yokota A., Kuo H. P. ( 2007 ). Reclassification of Bacillus axarquiensis Ruiz-Garcia et al. 2005 and Bacillus malacitensis Ruiz-Garcia et al. 2005 as later heterotypic synonyms of Bacillus mojavensis Roberts et al. 1994. . Int J Syst Evol Microbiol 57, 16631667. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.070938-0
Loading
/content/journal/ijsem/10.1099/ijs.0.070938-0
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error