1887

Abstract

A Gram-negative, spiral-shaped, chemolithotrophic, ammonia-oxidizing bacterium, designated APG3, was isolated into pure culture from sandy lake sediment collected from Green Lake, Seattle, WA, USA. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain APG3 belongs to cluster 0 of the genus , which is presently not represented by described species, with (cluster 3) as the closest species with a validly published name (identity of 98.6 % to the type strain). Strain APG3 grew at 4 °C but could not grow at 35 °C, indicating that this bacterium is psychrotolerant. Remarkably, the strain was able to grow over a wide range of pH (pH 5–9), which was greater than the pH range of any studied ammonia-oxidizing bacteria in pure culture. The DNA G+C content of the APG3 genome is 53.5 %, which is similar to that of ATCC 25196 (53.9 %) but higher than that of ATCC 19718 (50.7 %) and C71 (48.5 %). The average nucleotide identity (ANI) calculated for the genomes of strain APG3 and ATCC 25196 was 75.45 %, significantly lower than the value of 95 % ANI that corresponds to the 70 % species-level cut-off based on DNA–DNA hybridization. Overall polyphasic taxonomy study indicated that strain APG3 represents a novel species in the genus , for which the name sp. nov. is proposed (type strain APG3 = NCIMB 14869 = LMG 27536 = ATCC BAA-2542).

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2015-01-01
2019-11-15
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