1887

Abstract

A facultatively anaerobic, Gram-stain-negative, non-motile and rod-shaped bacterium, strain N-10, was isolated from the gut of the termite . Strain N-10 was closely related to JCM 16698 according to 16S rRNA gene sequence similarity analysis (98 %) and DNA–DNA relatedness value (≤61.3 %). The optimum growth temperature of strain N-10 was 30 °C, which was distinct from that (37 °C) of known species of the genus . Growth of strain N-10 was inhibited on medium containing 5 or 20 % bile, unlike other species of the genus . In addition, acid production in the API 20A system and enzymic reactions in the Rapid ID 32A system of strain N-10 differed from those of other species of the genus . Based on these characteristics, strain N-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N-10 ( = JCM 30204 = CCUG 66188).

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2015-02-01
2020-08-12
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References

  1. Brune A. ( 2014 ). Symbiotic digestion of lignocellulose in termite guts. . Nat Rev Microbiol 12, 168180. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid – deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  4. Hofstad T., Olsen I., Eribe E. R., Falsen E., Collins M. D., Lawson P. A. ( 2000 ). Dysgonomonas gen. nov. to accommodate Dysgonomonas gadei sp. nov., an organism isolated from a human gall bladder, and Dysgonomonas capnocytophagoides (formerly CDC group DF-3). . Int J Syst Evol Microbiol 50, 21892195. [CrossRef] [PubMed]
    [Google Scholar]
  5. Holdeman L. V., Cato E. P., Moore W. E. C. ( 1977 ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  6. Hongoh Y. ( 2011 ). Toward the functional analysis of uncultivable, symbiotic microorganisms in the termite gut. . Cell Mol Life Sci 68, 13111325. [CrossRef] [PubMed]
    [Google Scholar]
  7. Inoue T., Murashima K., Azuma J.-I., Sugimoto A., Slaytor M. ( 1997 ). Cellulose and xylan utilisation in the lower termite Reticulitermes speratus . . Insect Physiol 43, 235242. [CrossRef]
    [Google Scholar]
  8. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [CrossRef]
    [Google Scholar]
  10. McClung L. S., Lindberg R. B. ( 1957 ). The study of obligately anaerobic bacteria. . In Manual of Microbiological Methods, pp. 120139. Edited by Pelczar M. J. . New York:: McGraw-Hill;.
    [Google Scholar]
  11. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  12. Nakajima H., Hongoh Y., Usami R., Kudo T., Ohkuma M. ( 2005 ). Spatial distribution of bacterial phylotypes in the gut of the termite Reticulitermes speratus and the bacterial community colonizing the gut epithelium. . FEMS Microbiol Ecol 54, 247255. [CrossRef] [PubMed]
    [Google Scholar]
  13. Ohkuma M., Noda S., Hongoh Y., Kudo T. ( 2002 ). Diverse bacteria related to the bacteroides subgroup of the CFB phylum within the gut symbiotic communities of various termites. . Biosci Biotechnol Biochem 66, 7884. [CrossRef] [PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  15. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002 ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52, 841849. [CrossRef] [PubMed]
    [Google Scholar]
  16. Shah H. N. ( 1992 ). The genus Bacteroides and related taxa. . In The Prokaryotes, , 2nd edn., pp. 35933607. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33, 152155.
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [CrossRef]
    [Google Scholar]
  19. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [CrossRef]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yang Y. J., Zhang N., Ji S. Q., Lan X., Zhang K. D., Shen Y. L., Li F. L., Ni J. F. ( 2014 ). Dysgonomonas macrotermitis sp. nov., isolated from the hindgut of a fungus-growing termite. . Int J Syst Evol Microbiol 64, 29562961. [CrossRef] [PubMed]
    [Google Scholar]
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