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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_2

sp. nov., isolated from the root of Free

Abstract

A yellowish-pigmented bacterium, designated strain PLGR-1, was isolated from the root of collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain PLGR-1 were Gram-stain-negative, strictly aerobic, rod-shaped, non-spore-forming and motile with a single polar flagellum. Growth occurred at 7–33 °C (optimum, 25–28 °C), at pH 5.0–10.0 (optimum, pH 6.0–7.0) and with 0–0.5 % (w/v) NaCl (optimum, 0 %). The predominant respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids were summed feature 3 (comprising Cω7 and/or Cω6), C and summed feature 8 (comprising Cω7 and/or Cω6). The major polyamines were putrescine and 2-hydroxyputrescine and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA GC content was 69.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLGR-1 belonged to the class and formed a tight phyletic lineage with members of the genus . Strain PLGR-1 was most closely related to DSM 11587 and DSM 19916, with 16S rRNA gene sequence similarities of 98.5 and 98.0 %, respectively. The DNA–DNA relatedness values between strain PLGR-1 and the type strains of and were 46.3 and 14.7 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain PLGR-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PLGR-1 ( = CCTCC AB 2013018 = KCTC 32299 = LMG 27607).

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Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2013AA102802)
  • National Natural Science Foundation of China (Award 31100001 and 31300158)
  • Natural Science Foundation of Shaanxi Province, China (Award 2012JQ3006)
  • Fundamental Research Funds for the Central Universities (Award QN2012020)
  • Specialized Research Fund for the Doctoral Program of Higher Education of China (Award 20120204120032)
© 2015 IUMS
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2015-02-01
2025-01-24
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Rhizobacter bergeniae sp. nov., isolated from the root of Bergenia scopulosa
Int J Syst Evol Microbiol 65, 479 (2015); https://doi.org/10.1099/ijs.0.070318-0
/content/journal/ijsem/10.1099/ijs.0.070318-0
/content/journal/ijsem/10.1099/ijs.0.070318-0
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