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Abstract

A Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8 was related most closely to the genus and shared highest sequence similarity of 97.7 % with EM44. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C, iso-C G and iso-C 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8 included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8 and EM44 were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8 ( = CGMCC 1.12606 = KCTC 32436) is considered to be the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2015-02-01
2020-01-20
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