sp. nov., isolated from a marine sponge and emended description of the genus Wiese 2009 and Free

Abstract

A Gram-reaction-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC09566 was isolated from a sponge collected at Uljin County in the coastal area of the East Sea (36° 55′ N, 129° 25′ E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC09566 showed the highest similarity with the LD81 (96.7 %). Growth was observed at 11–31 °C (optimum 25 °C), at pH 6.0–8.5 (optimum pH 7.0) and with 0–6 % (optimum 2.5 %) NaCl. The predominant cellular fatty acids were summed feature 8 (comprised of Cω7/Cω6) and summed feature 3 (comprised of Cω7 and/or Cω6). The DNA G+C content is 44.6 mol%. The major respiratory quinone is Q-9. Phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminophospholipids and one unidentified aminolipid were detected as major polar lipids. On the basis of this polyphasic taxonomic data, it is concluded that strain MEBiC09566 should be classified as representing a novel species in the genus and the name proposed is sp. nov. The type strain is MEBiC09566 ( = KCCM 43040 = JCM 19930). Emended descriptions of the genus Wiese 2009 and are also given.

Funding
This study was supported by the:
  • Korean Institute of Ocean Science and Technology (KIOST) (Award PE99212)
  • Marine BioResource Bank Program of the Ministry of Ocean & Fisheries, Korea
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2015-01-01
2024-03-29
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