%0 Journal Article %A Amoozegar, Mohammad Ali %A Makhdoumi-Kakhki, Ali %A Mehrshad, Maliheh %A Rasooli, Mehrnoosh %A Fazeli, Seyed Abolhassan Shahzadeh %A Spröer, Cathrin %A Ventosa, Antonio %T Halovivax cerinus sp. nov., an extremely halophilic archaeon from a hypersaline lake %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_1 %P 65-70 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.069757-0 %I Microbiology Society, %X An extremely halophilic archaeon, strain IC35T, was isolated from a mud sample of the Aran-Bidgol salt lake in Iran. The novel strain was cream, non-motile, rod-shaped and required at least 2.5 M NaCl, but not MgCl2, for growth. Optimal growth was achieved with 3.4 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.0 (grew over a pH range of 6.5–9.0) and 40 °C (grew over a temperature range of 30–50 °C), respectively. Analysis of 16S rRNA gene sequences revealed that strain IC35T clustered with species of the genus Halovivax , with sequence similarities of 97.3 %, 96.6 % and 96.3 %, respectively, to Halovivax limisalsi IC38T, Halovivax asiaticus EJ-46T and Halovivax ruber XH-70T. The rpoB′ gene similarities between the novel strain and Halovivax limisalsi IBRC-M 10022T, Halovivax ruber JCM 13892T and Halovivax asiaticus JCM 14624T were 90.2 %, 90.2 % and 89.9 %, respectively. The polar lipid pattern of strain IC35T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; six unknown glycolipids and two minor phospholipids were also observed. The only quinone present was MK-8 (II-H2). The G+C content of the genomic DNA was 63.2 mol%. DNA–DNA hybridization studies (29 % hybridization with Halovivax limisalsi IBRC-M 10022T), as well as biochemical and physiological characterization, allowed strain IC35T to be differentiated from other species of the genus Halovivax . A novel species, Halovivax cerinus sp. nov., is therefore proposed to accommodate this strain. The type strain is IC35T ( = IBRC-M 10256T = KCTC 4050T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.069757-0