%0 Journal Article %A Lee, Jae-Chan %A Kim, Song-Gun %A Whang, Kyung-Sook %T Sphingobium subterraneum sp. nov., isolated from ground water %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_2 %P 393-398 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.069708-0 %I Microbiology Society, %X A Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, yellow, rod-shaped bacterium, designated S-II-13T, was isolated from ground water at Daejeon in Korea. Strain S-II-13T grew between 15 and 30 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0.3–1.5 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain S-II-13T was found to belong to the genus Sphingobium , showing closest phylogenetic similarity to Rhizorhapis suberifaciens CA1T (97.0 % 16S rRNA gene sequence similarity), Sphingobium sufflavum HL-25T (96.9 %) and Sphingobium vulgare HU1-GD12T (96.6 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone was Q-10. The major fatty acids were C18 : 1ω7c, C14 : 0 2-OH, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain S-II-13T was 63.5 mol%. DNA–DNA relatedness between strain S-II-13T and Rhizorhapis suberifaciens LMG 17323T, Sphingobium sufflavum KCTC 23953T and Sphingobium vulgare KCTC 22289T was 24, 52 and 55 %, respectively. On the basis of evidence from this taxonomic study using a polyphasic approach, strain S-II-13T represents a novel species of the genus Sphingobium for which the name Sphingobium subterraneum sp. nov. is proposed. The type strain is S-II-13T ( = KACC 17606T = NBRC 109814T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.069708-0