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Volume 65, Issue Pt_2

sp. nov., isolated from ground water Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, yellow, rod-shaped bacterium, designated S-II-13, was isolated from ground water at Daejeon in Korea. Strain S-II-13 grew between 15 and 30 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0.3–1.5 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain S-II-13 was found to belong to the genus , showing closest phylogenetic similarity to CA1 (97.0 % 16S rRNA gene sequence similarity), HL-25 (96.9 %) and HU1-GD12 (96.6 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone was Q-10. The major fatty acids were Cω7, C 2-OH, C and summed feature 3 (Cω7 and/or iso-C 2-OH). The DNA G+C content of strain S-II-13 was 63.5 mol%. DNA–DNA relatedness between strain S-II-13 and LMG 17323, KCTC 23953 and KCTC 22289 was 24, 52 and 55 %, respectively. On the basis of evidence from this taxonomic study using a polyphasic approach, strain S-II-13 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is S-II-13 ( = KACC 17606 = NBRC 109814).

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Funding
This study was supported by the:
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
© 2015 IUMS
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2015-02-01
2024-04-19
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Sphingobium subterraneum sp. nov., isolated from ground water
Int J Syst Evol Microbiol 65, 393 (2015); https://doi.org/10.1099/ijs.0.069708-0
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