1887

Abstract

Strain RK1, a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium was isolated from a hexachlorocyclohexane (HCH) dumpsite, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RK1 belongs to the family and showed highest sequence similarity to Jip14 (95.63 %). The major cellular fatty acids of strain RK1 were iso-C, summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH, summed feature 9 (10-methyl C and/or iso-Cω9), iso-C 3-OH and C. The major respiratory pigment and polyamine of RK1 were menaquinone (MK-7) and homospermidine, respectively. The main polar lipids were phosphatidylethanolamine and sphingolipid. The G+C content of the DNA was 44.5 mol%. The results of physiological and biochemical tests and 16S rRNA sequence analysis clearly demonstrated that strain RK1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RK1 ( = DSM 28470 = MCC 2546).

Funding
This study was supported by the:
  • Department of Biotechnology (DBT), Government of India (Award BT/PR3301/BCE/8/875/11)
  • All India Network Project on Soil Biodiversity-Biofertilizer (ICAR), Department of Science and Technology (Award SR/SO/AS-24/2011)
  • University of Delhi/Department of Science and Technology (Award DU-DST—PURSE)
  • Indian Council for Agricultral Research (ICAR)
  • University Grant Commission (UGC)
  • Council for Scientific and Industrial Research (CSIR)
  • Department of Biotechnology (DBT), Government of India
  • All India Network Project on Soil Biodiversity-Biofertilizer (ICAR)
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2015-01-01
2024-11-08
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