1887

Abstract

A new Gram-staining-positive, non-sporulating, mesophilic, amino acid-degrading anaerobic bacterium, designated strain OTA 102, was isolated from an anaerobic sequencing batch reactor treating wastewater from cooking tuna. The cells were curved rods (0.6–2.5×0.5 µm) and occurred singly or in pairs. The strain was motile by means of one lateral flagellum. Strain OTA 102 grew at temperatures between 30 and 45 °C (optimum 40 °C), between pH 6.0 and 8.4 (optimum pH 7.2) and NaCl concentrations between 1 and 5 % (optimum 2 %, w/v). Strain OTA 102 required yeast extract for growth. Serine, threonine, glycine, cysteine, citrate, fumarate, α-ketoglutarate and pyruvate were fermented. When co-cultured with as the hydrogen scavenger, strain OTA 102 oxidized alanine, valine, leucine, isoleucine, aspartate, tyrosine, methionine, histidine and asparagine. The genomic DNA G+C content of strain OTA 102 was 41.7 mol%. The main fatty acid was iso-C. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain OTA 102 was related to and (95.5 and 95.2 % similarity, respectively), of the phylum . On the basis of phylogenetic, genetic and physiological characteristics, strain OTA 102 is proposed to represent a novel species of the genus , sp. nov. The type strain is OTA 102 ( = DSM 27500 = JCM 19320).

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2015-02-01
2021-10-20
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  2. Baena S., Fardeau M. L., Labat M., Ollivier B., Thomas P., Garcia J. L., Patel B. K. C. ( 1998 ). Aminobacterium colombiense gen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge. . Anaerobe 4, 241250. [View Article] [PubMed]
    [Google Scholar]
  3. Baena S., Fardeau M. L., Ollivier B., Labat M., Thomas P., Garcia J. L., Patel B. K. ( 1999a ). Aminomonas paucivorans gen. nov., sp. nov., a mesophilic, anaerobic, amino-acid-utilizing bacterium. . Int J Syst Bacteriol 49, 975982. [View Article] [PubMed]
    [Google Scholar]
  4. Baena S., Fardeau M. L., Woo T. H. S., Ollivier B., Labat M., Patel B. K. C. ( 1999b ). Phylogenetic relationships of three amino-acid-utilizing anaerobes, Selenomonas acidaminovorans, ‘Selenomonas acidaminophila’ and Eubacterium acidaminophilum, as inferred from partial 16S rDNA nucleotide sequences and proposal of Thermanaerovibrio acidaminovorans gen. nov., comb. nov. and Anaeromusa acidaminophila gen. nov., comb. nov.. Int J Syst Bacteriol 49, 969974. [View Article] [PubMed]
    [Google Scholar]
  5. Baena S., Fardeau M.-L., Labat M., Ollivier B., Garcia J.-L., Patel B. K. C. ( 2000 ). Aminobacterium mobile sp. nov., a new anaerobic amino-acid-degrading bacterium. . Int J Syst Evol Microbiol 50, 259264. [View Article] [PubMed]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  7. Chia L.-J., Wen C.-K. ( 2002 ). Utilization of cooking juice young tuna processed into cannel tuna as condiments: effect of enzymatic hydrolysis and membrane treatment. . Fish Sci 68, 13441351. [View Article]
    [Google Scholar]
  8. Debroas D., Blanchart G. ( 1993 ). Interactions between proteolytic and cellulolytic rumen bacteria during hydrolysis of plant cell wall protein. . Reprod Nutr Dev 33, 283288. [View Article] [PubMed]
    [Google Scholar]
  9. Elsden S. R., Hilton M. G. ( 1979 ). Amino acid utilization patterns in clostridial taxonomy. . Arch Microbiol 123, 137141. [View Article] [PubMed]
    [Google Scholar]
  10. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F. S., Garcia J.-L. ( 1997 ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. . Int J Syst Bacteriol 47, 10131019. [View Article] [PubMed]
    [Google Scholar]
  11. Fardeau M.-L., Magot M., Patel B. K. C., Thomas P., Garcia J.-L., Ollivier B. ( 2000 ). Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. . Int J Syst Evol Microbiol 50, 21412149. [View Article] [PubMed]
    [Google Scholar]
  12. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783787. [View Article]
    [Google Scholar]
  13. Godon J. J., Morinière J., Moletta M., Gaillac M., Bru V., Delgènes J. P. ( 2005 ). Rarity associated with specific ecological niches in the bacterial world: the ‘Synergistes’ example. . Environ Microbiol 7, 213224. [View Article] [PubMed]
    [Google Scholar]
  14. Honda T., Fujita T., Tonouchi A. ( 2013 ). Aminivibrio pyruvatiphilus gen. nov., sp. nov., an anaerobic, amino-acid-degrading bacterium from soil of a Japanese rice field. . Int J Syst Evol Microbiol 63, 36793686. [View Article] [PubMed]
    [Google Scholar]
  15. Hungate R. E. ( 1969 ). A roll tube method for the cultivation of strict anaerobes. . Methods Microbiol 3B, 117132. [View Article]
    [Google Scholar]
  16. Jumas-Bilak E., Carlier J. P., Jean-Pierre H., Citron D., Bernard K., Damay A., Gay B., Teyssier C., Campos J., Marchandin H. ( 2007 ). Jonquetella anthropi gen. nov., sp. nov., the first member of the candidate phylum ‘Synergistetes’ isolated from man. . Int J Syst Evol Microbiol 57, 27432748. [View Article] [PubMed]
    [Google Scholar]
  17. Jumas-Bilak E., Roudière L., Marchandin H. ( 2009 ). Description of ‘Synergistetes’ phyl. nov. and emended description of the phylum ‘Deferribacteres’ and of the family Syntrophomonadaceae, phylum ‘Firmicutes’. . Int J Syst Evol Microbiol 59, 10281035. [View Article] [PubMed]
    [Google Scholar]
  18. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and desoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  19. Magot M., Ravot G., Campaignolle X., Ollivier B., Patel B. K., Fardeau M. L., Thomas P., Crolet J. L., Garcia J. L. ( 1997 ). Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. . Int J Syst Bacteriol 47, 818824. [View Article] [PubMed]
    [Google Scholar]
  20. Maune M. W., Tanner R. S. ( 2012 ). Description of Anaerobaculum hydrogeniformans sp. nov., an anaerobe that produces hydrogen from glucose, and emended description of the genus Anaerobaculum . . Int J Syst Evol Microbiol 62, 832838. [View Article] [PubMed]
    [Google Scholar]
  21. Mead G. C. ( 1971 ). The amino acid-fermenting clostridia. . J Gen Microbiol 67, 4756. [View Article] [PubMed]
    [Google Scholar]
  22. Menes R. J., Muxí L. ( 2002 ). Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum . . Int J Syst Evol Microbiol 52, 157164.[PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  24. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  25. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [View Article] [PubMed]
    [Google Scholar]
  26. Rees G. N., Patel B. K., Grassia G. S., Sheehy A. J. ( 1997 ). Anaerobaculum thermoterrenum gen. nov., sp. nov., a novel, thermophilic bacterium which ferments citrate. . Int J Syst Bacteriol 47, 150154. [View Article] [PubMed]
    [Google Scholar]
  27. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  29. Thabet O. B., Fardeau M.-L., Joulian C., Thomas P., Hamdi M., Garcia J.-L., Ollivier B. ( 2004 ). Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse. . Anaerobe 10, 185190. [View Article] [PubMed]
    [Google Scholar]
  30. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2007 ). The division “Synergistes” . . Anaerobe 13, 99106. [View Article] [PubMed]
    [Google Scholar]
  31. Widdel F., Pfennig N. ( 1982 ). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov.. Arch Microbiol 131, 360365. [View Article]
    [Google Scholar]
  32. Zavarzina D. G., Zhilina T. N., Tourova T. P., Kuznetsov B. B., Kostrikina N. A., Bonch-Osmolovskaya E. A. ( 2000 ). Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio . . Int J Syst Evol Microbiol 50, 12871295. [View Article] [PubMed]
    [Google Scholar]
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