1887

Abstract

A new Gram-staining-positive, non-sporulating, mesophilic, amino acid-degrading anaerobic bacterium, designated strain OTA 102, was isolated from an anaerobic sequencing batch reactor treating wastewater from cooking tuna. The cells were curved rods (0.6–2.5×0.5 µm) and occurred singly or in pairs. The strain was motile by means of one lateral flagellum. Strain OTA 102 grew at temperatures between 30 and 45 °C (optimum 40 °C), between pH 6.0 and 8.4 (optimum pH 7.2) and NaCl concentrations between 1 and 5 % (optimum 2 %, w/v). Strain OTA 102 required yeast extract for growth. Serine, threonine, glycine, cysteine, citrate, fumarate, α-ketoglutarate and pyruvate were fermented. When co-cultured with as the hydrogen scavenger, strain OTA 102 oxidized alanine, valine, leucine, isoleucine, aspartate, tyrosine, methionine, histidine and asparagine. The genomic DNA G+C content of strain OTA 102 was 41.7 mol%. The main fatty acid was iso-C. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain OTA 102 was related to and (95.5 and 95.2 % similarity, respectively), of the phylum . On the basis of phylogenetic, genetic and physiological characteristics, strain OTA 102 is proposed to represent a novel species of the genus , sp. nov. The type strain is OTA 102 ( = DSM 27500 = JCM 19320).

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2015-02-01
2019-10-15
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