1887

Abstract

A Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6, was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15–35 °C (optimum 30 °C), pH 4.0–10.0 (optimum pH 7.0) and 0–3.0 % (w/v) NaCl (optimum 1.0 %). Phylogenetic analysis based on 16S rRNA sequences showed that strain BUT-6 was most closely related to PYM5-11 (98.6 % similarity). However, the DNA–DNA relatedness between strain BUT-6 and PYM5-11 was 47.1 %. The major fatty acids (>10 % of total fatty acids) of strain BUT-6 were iso-C, iso-Cω9 and iso-C. The major respiratory quinone was ubiquinone Q-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6 was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BUT-6 ( = CCTCC AB 2013266 = KACC 17139).

Funding
This study was supported by the:
  • National Science and Technology Support Plan (Award 2013AA102804)
  • National Natural Science Foundation of China (Award Grant no. 31270157)
  • Program for New Century Excellent Talents in University (Award NCET-13-0861)
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2015-02-01
2021-10-19
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