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Volume 64, Issue Pt_11

sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Jang 2011 to the genus as comb. nov. Free

Abstract

A Gram-staining-negative, facultatively aerobic bacterium, designated SM-2, was isolated from a sea-tidal flat of Yellow Sea, South Korea. Cells were catalase- and oxidase-positive motile rods with a single polar flagellum. Growth of strain SM-2 was observed at 10–37 °C (optimum, 25–30 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5) and in the presence of 0–11 % (w/v) NaCl (optimum, 2 %). Strain SM-2 contained ubiquinone-8 (Q-8) as the sole isoprenoid quinone and Cω8, summed feature 3 (comprising Cω7 and/or iso-C 2-OH), C and Cω7 as the major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified lipid were identified as the major cellular polar lipids. The G+C content of the genomic DNA was 52.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SM-2 formed a tight phyletic lineage with ZS5-23, ZS6-22 and CL-AS9, but that CL-AS9 was distinct from other species of the genus . Based on 16S rRNA gene sequence similarities, strain SM-2 was most closely related to CL-AS9, ZS5-23 and ZS6-22, with similarities of 99.5 %, 98.9 % and 98.7 %, respectively, but the DNA–DNA hybridization values among these species were clearly lower than 70 %. On the basis of chemotaxonomic data and molecular properties, we propose strain SM-2 represents a novel species of the genus with the name sp. nov. (type strain SM-2 = KACC 18120 = JCM 30138). We also propose the transfer of Jang 2011 to the genus as comb. nov. (type strain CL-AS9 = KCCM 90094 = JCM 17304).

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Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR) (Award 2014-02-066)
© 2014 IUMS
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2014-11-01
2024-04-23
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Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov.
Int J Syst Evol Microbiol 64, 3768 (2014); https://doi.org/10.1099/ijs.0.068593-0
/content/journal/ijsem/10.1099/ijs.0.068593-0
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