A Gram-reaction-negative, aerobic, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, designated W-SW2T, was isolated from seawater in the South Sea of South Korea. The novel strain grew optimally at pH 7.0–8.0, at 25 °C and in the presence of approximately 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain W-SW2T fell within the clade comprising the type strains of species of the genus Loktanella, clustering and sharing the highest sequence similarity value (96.3 %) with the type strain of Loktanella koreensis. The 16S rRNA gene sequence similarity values between strain W-SW2T and the type strains of the other species of the genus Loktanella were in the range 93.1–96.0 %. The DNA G+C content of strain W-SW2T was 55.9 mol%. Strain W-SW2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain W-SW2T is separated phylogenetically from other species of the genus Loktanella. On the basis of the data presented, strain W-SW2T is considered to represent a novel species of the genus Loktanella, for which the name Loktanella ponticola sp. nov. is proposed. The type strain is W-SW2T ( = KCTC 42133T = NBRC 110409T).
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Funding
This study was supported by the:
National Institute of Biological Resources (NIBR)
Ministry of Environment (MOE)
Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
(Award NRF-2013M3A9A5075953)
BarrowG. I.,
FelthamR. K. A.(editors) (1993).Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
BaumannP.,
BaumannL.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes
. . In The Prokaryotes, pp. 1302–1331. Edited by
StarrM. P.,
StolpH.,
TrüperH. G.,
BalowsA.,
SchlegelH. G.
. Berlin:: Springer;.
BrunsA.,
RohdeM.,
Berthe-CortiL.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol51, 1997–2006. [View Article][PubMed]
EmbleyT. M.,
WaitR.(1994). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by
GoodfellowM.,
O’DonnellA. G.
. Chichester:: Wiley;.
EuzébyJ. P.(1997). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol47, 590–592. http://www.bacterio.net [View Article][PubMed]
LauS. C. K.,
TsoiM. M. Y.,
LiX.,
PlakhotnikovaI.,
WuM.,
WongP.-K.,
QianP.-Y.(2004).Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. . Int J Syst Evol Microbiol54, 2281–2284. [View Article][PubMed]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
MoonY. G.,
SeoS. H.,
LeeS. D.,
HeoM. S.(2010).Loktanella pyoseonensis sp. nov., isolated from beach sand, and emended description of the genus Loktanella
. . Int J Syst Evol Microbiol60, 785–789. [View Article][PubMed]
ParkS.,
LeeJ.-S.,
LeeK.-C.,
YoonJ.-H.(2013b).Loktanella soesokkakensis sp. nov., isolated from the junction between the North Pacific Ocean and a freshwater spring. . Antonie van Leeuwenhoek104, 397–404. [View Article][PubMed]
StackebrandtE.,
GoebelB. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol44, 846–849. [View Article]
TamaokaJ.,
KomagataK.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25, 125–128. [View Article]
TsubouchiT.,
ShimaneY.,
MoriK.,
MiyazakiM.,
TameA.,
UematsuK.,
MaruyamaT.,
HatadaY.(2013).Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella
. . Int J Syst Evol Microbiol63, 1390–1395. [View Article][PubMed]
Van TrappenS.,
MergaertJ.,
SwingsJ.(2004).Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. . Int J Syst Evol Microbiol54, 1263–1269. [View Article][PubMed]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfellowM.,
ParkY.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol46, 502–505. [View Article]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol48, 187–194. [View Article][PubMed]
YoonJ.-H.,
KangK. H.,
ParkY.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol53, 449–454. [View Article][PubMed]
YoonJ.-H.,
KangS.-J.,
LeeS.-Y.,
OhT.-K.(2007).Loktanella maricola sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol57, 1799–1802. [View Article][PubMed]