sp. nov., isolated from seawater Free

Abstract

A Gram-reaction-negative, aerobic, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, designated W-SW2, was isolated from seawater in the South Sea of South Korea. The novel strain grew optimally at pH 7.0–8.0, at 25 °C and in the presence of approximately 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain W-SW2 fell within the clade comprising the type strains of species of the genus , clustering and sharing the highest sequence similarity value (96.3 %) with the type strain of . The 16S rRNA gene sequence similarity values between strain W-SW2 and the type strains of the other species of the genus were in the range 93.1–96.0 %. The DNA G+C content of strain W-SW2 was 55.9 mol%. Strain W-SW2 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain W-SW2 is separated phylogenetically from other species of the genus . On the basis of the data presented, strain W-SW2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W-SW2 ( = KCTC 42133 = NBRC 110409).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE)
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award NRF-2013M3A9A5075953)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.068361-0
2014-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3717.html?itemId=/content/journal/ijsem/10.1099/ijs.0.068361-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 2568. [View Article]
    [Google Scholar]
  5. Embley T. M., Wait R. ( 1994 ). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121161. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  6. Euzéby J. P. ( 1997 ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590592. http://www.bacterio.net [View Article] [PubMed]
    [Google Scholar]
  7. Hosoya S., Yokota A. ( 2007 ). Loktanella atrilutea sp. nov., isolated from seawater in Japan. . Int J Syst Evol Microbiol 57, 19661969. [View Article] [PubMed]
    [Google Scholar]
  8. Ivanova E. P., Zhukova N. V., Lysenko A. M., Gorshkova N. M., Sergeev A. F., Mikhailov V. V., Bowman J. P. ( 2005 ). Loktanella agnita sp. nov. and Loktanella rosea sp. nov., from the north-west Pacific Ocean. . Int J Syst Evol Microbiol 55, 22032207. [View Article] [PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  10. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  11. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Wu M., Wong P.-K., Qian P.-Y. ( 2004 ). Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. . Int J Syst Evol Microbiol 54, 22812284. [View Article] [PubMed]
    [Google Scholar]
  12. Lee S. D. ( 2012 ). Loktanella tamlensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62, 586590. [View Article] [PubMed]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  14. Moon Y. G., Seo S. H., Lee S. D., Heo M. S. ( 2010 ). Loktanella pyoseonensis sp. nov., isolated from beach sand, and emended description of the genus Loktanella . . Int J Syst Evol Microbiol 60, 785789. [View Article] [PubMed]
    [Google Scholar]
  15. Park S., Jung Y.-T., Yoon J.-H. ( 2013a ). Loktanella sediminilitoris sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 63, 41184123. [View Article] [PubMed]
    [Google Scholar]
  16. Park S., Lee J.-S., Lee K.-C., Yoon J.-H. ( 2013b ). Loktanella soesokkakensis sp. nov., isolated from the junction between the North Pacific Ocean and a freshwater spring. . Antonie van Leeuwenhoek 104, 397404. [View Article] [PubMed]
    [Google Scholar]
  17. Park J.-M., Park S. Y., Jung Y.-T., Cho J. Y., Yoon J.-H. ( 2014 ). Loktanella variabilis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol doi:10.1099/ijs.0.065417-0 [Epub ahead of print]. [View Article] [PubMed]
    [Google Scholar]
  18. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  20. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  21. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  22. Tanaka N., Romanenko L. A., Kurilenko V. V., Svetashev V. I., Kalinovskaya N. I., Mikhailov V. V. ( 2014 ). Loktanella maritima sp. nov. isolated from shallow marine sediments. . Int J Syst Evol Microbiol 64, 23702375. [View Article] [PubMed]
    [Google Scholar]
  23. Tsubouchi T., Shimane Y., Mori K., Miyazaki M., Tame A., Uematsu K., Maruyama T., Hatada Y. ( 2013 ). Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella . . Int J Syst Evol Microbiol 63, 13901395. [View Article] [PubMed]
    [Google Scholar]
  24. Van Trappen S., Mergaert J., Swings J. ( 2004 ). Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. . Int J Syst Evol Microbiol 54, 12631269. [View Article] [PubMed]
    [Google Scholar]
  25. Weon H.-Y., Kim B.-Y., Yoo S.-H., Kim J.-S., Kwon S.-W., Go S.-J., Stackebrandt E. ( 2006 ). Loktanella koreensis sp. nov., isolated from sea sand in Korea. . Int J Syst Evol Microbiol 56, 21992202. [View Article] [PubMed]
    [Google Scholar]
  26. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  27. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  28. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003 ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53, 449454. [View Article] [PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K. ( 2007 ). Loktanella maricola sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57, 17991802. [View Article] [PubMed]
    [Google Scholar]
  30. Yoon J.-H., Jung Y.-T., Lee J.-S. ( 2013 ). Loktanella litorea sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 63, 175180. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.068361-0
Loading
/content/journal/ijsem/10.1099/ijs.0.068361-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed