Full text loading...
Abstract
A Gram-reaction-negative, aerobic, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, designated W-SW2T, was isolated from seawater in the South Sea of South Korea. The novel strain grew optimally at pH 7.0–8.0, at 25 °C and in the presence of approximately 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain W-SW2T fell within the clade comprising the type strains of species of the genus Loktanella , clustering and sharing the highest sequence similarity value (96.3 %) with the type strain of Loktanella koreensis . The 16S rRNA gene sequence similarity values between strain W-SW2T and the type strains of the other species of the genus Loktanella were in the range 93.1–96.0 %. The DNA G+C content of strain W-SW2T was 55.9 mol%. Strain W-SW2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain W-SW2T is separated phylogenetically from other species of the genus Loktanella . On the basis of the data presented, strain W-SW2T is considered to represent a novel species of the genus Loktanella , for which the name Loktanella ponticola sp. nov. is proposed. The type strain is W-SW2T ( = KCTC 42133T = NBRC 110409T).
- Published Online:
Funding
- National Institute of Biological Resources (NIBR)
- Ministry of Environment (MOE)
- Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award NRF-2013M3A9A5075953)