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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_11

gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium Free

Abstract

A bacterial strain designated SBNa008 was isolated from a Philippine soil sample. It exhibited the general characteristics associated with myxobacteria, such as swarming of Gram-negative vegetative rod cells, fruiting body and myxospore formation and predatory behaviour in lysing micro-organisms. The novel strain was characterized as mesophilic, chemoheterotrophic and aerobic. The major fatty acids were Cω6,9,12,15 all (arachidonic acid), iso-C, C 2-OH and iso-C dimethylacetal. Interestingly, SBNa008 contained diverse fatty acids belonging to the commercially valuable polyunsaturated omega-6 and omega-3 families, and a highly conjugated dihydroxylated C steroid. The G+C content of the genomic DNA was 67.3 mol%. The 16S rRNA gene sequence revealed 95–96 % similarity to sequences derived from clones of uncultured bacteria and 94–95 % similarity to cultured members of the suborder . Phylogenetic analysis revealed that strain SBNa008 formed a novel lineage in the suborder . Based on a polyphasic taxonomic characterization, we propose that strain SBNa008 represents a novel genus and species, gen. nov., sp. nov. The type strain of is SBNa008 ( = DSM 24000 = NCCB 100349).

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2014-11-01
2024-04-23
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References

  1. Bode H. B., Zeggel B., Silakowski B., Wenzel S. C., Reichenbach H., Müller R. ( 2003 ). Steroid biosynthesis in prokaryotes: identification of myxobacterial steroids and cloning of the first bacterial 2,3(S)-oxidosqualene cyclase from the myxobacterium Stigmatella aurantiaca . . Mol Microbiol 47, 471–481. [View Article] [PubMed]
    [Google Scholar]
  2. Dawid W. ( 2000 ). Biology and global distribution of myxobacteria in soils. . FEMS Microbiol Rev 24, 403–427. [View Article] [PubMed]
    [Google Scholar]
  3. Drummond A. J., Ashton B., Buxton S., Cheung M., Heled J., Kearse M., Moir R., Stones-Havas S., Sturrock S. & other authors ( 2010 ). Geneious Pro 5.0.2. http://www.geneious.com
  4. Edgar R. C. ( 2004 ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32, 1792–1797. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  6. Fudou R., Jojima Y., Iizuka T., Yamanaka S. ( 2002 ). Haliangium ochraceum gen. nov., sp. nov. and Haliangium tepidum sp. nov.: novel moderately halophilic myxobacteria isolated from coastal saline environments. . J Gen Appl Microbiol 48, 109–115. [View Article] [PubMed]
    [Google Scholar]
  7. Garcia R. O., Krug D., Müller R. ( 2009a ). Discovering natural products from myxobacteria with emphasis on rare producer strains in combination with improved analytical methods. . Methods Enzymol 458, 59–91. [View Article] [PubMed]
    [Google Scholar]
  8. Garcia R. O., Reichenbach H., Ring M. W., Müller R. ( 2009b ). Phaselicystis flava gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium, and the description of Phaselicystidaceae fam. nov.. Int J Syst Evol Microbiol 59, 1524–1530. [View Article] [PubMed]
    [Google Scholar]
  9. Garcia R., Gerth K., Stadler M., Dogma I. J. Jr, Müller R. ( 2010 ). Expanded phylogeny of myxobacteria and evidence for cultivation of the ‘unculturables’. . Mol Phylogenet Evol 57, 878–887. [View Article] [PubMed]
    [Google Scholar]
  10. Garcia R., Pistorius D., Stadler M., Müller R. ( 2011 ). Fatty acid-related phylogeny of myxobacteria as an approach to discover polyunsaturated omega-3/6 fatty acids. . J Bacteriol 193, 1930–1942. [View Article] [PubMed]
    [Google Scholar]
  11. Gawas D., Garcia R., Huch V., Müller R. ( 2011 ). A highly conjugated dihydroxylated C28 steroid from a myxobacterium. . J Nat Prod 74, 1281–1283. [View Article] [PubMed]
    [Google Scholar]
  12. Gemperlein K., Rachid S., Garcia R. O., Wenzel S. C., Müller R. ( 2014 ). Polyunsaturated fatty acid biosynthesis in myxobacteria: different PUFA synthases and their product diversity. . Chem Sci 5, 1733–1741. [View Article]
    [Google Scholar]
  13. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. (editors) ( 1981 ). Manual of Methods for General Bacteriology. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  14. Iizuka T., Jojima Y., Fudou R., Yamanaka S. ( 1998 ). Isolation of myxobacteria from the marine environment. . FEMS Microbiol Lett 169, 317–322. [View Article] [PubMed]
    [Google Scholar]
  15. Iizuka T., Jojima Y., Fudou R., Hiraishi A., Ahn J. W., Yamanaka S. ( 2003a ). Plesiocystis pacifica gen. nov., sp. nov., a marine myxobacterium that contains dihydrogenated menaquinone, isolated from the Pacific coasts of Japan. . Int J Syst Evol Microbiol 53, 189–195. [View Article] [PubMed]
    [Google Scholar]
  16. Iizuka T., Jojima Y., Fudou R., Tokura M., Hiraishi A., Yamanaka S. ( 2003b ). Enhygromyxa salina gen. nov., sp. nov., a slightly halophilic myxobacterium isolated from the coastal areas of Japan. . Syst Appl Microbiol 26, 189–196. [View Article] [PubMed]
    [Google Scholar]
  17. Iizuka T., Jojima Y., Hayakawa A., Fujii T., Yamanaka S. S., Fudou R. ( 2013 ). Pseudenhygromyxa salsuginis gen. nov., sp. nov., a myxobacterium isolated from an estuarine marsh. . Int J Syst Evol Microbiol 63, 1360–1369. [View Article] [PubMed]
    [Google Scholar]
  18. Jansen R., Nowak A., Kunze B., Reichenbach H., Höfle G. ( 1995 ). Four new carotenoids from Polyangium fumosum (myxobacteria): 3,3′,4,4′-tetradehydro-1,1′,2,2′-tetrahydro-1,1′-dihydroxy-ψ,ψ-carotene (di-O-demethylspirilloxanthin), its β-glucoside and glucoside fatty acid esters. . Liebigs Ann 1995, 873–876. [View Article]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  20. Kohl W., Gloe A., Reichenbach H. ( 1983 ). Steroids from the myxobacterium Nannocystis exedens . . J Gen Microbiol 129, 1629–1635.
     [Google Scholar]
  21. Lachnik J., Ackermann B., Bohrssen A., Maass S., Diephaus C., Puncken A., Stermann M., Bange F.-C. ( 2002 ). Rapid-cycle PCR and fluorimetry for detection of mycobacteria. . J Clin Microbiol 40, 3364–3373. [View Article] [PubMed]
    [Google Scholar]
  22. Li G., Shimelis O., Zhou X., Giese R. W. ( 2003 ). Scaled-down nuclease P1 for scaled-up DNA digestion. . Biotechniques 34, 908–909.[PubMed]
    [Google Scholar]
  23. McCurdy H. D. ( 1969 ). Studies on the taxonomy of the Myxobacterales. I. Record of Canadian isolates and survey of methods. . Can J Microbiol 15, 1453–1461. [View Article] [PubMed]
    [Google Scholar]
  24. Mohr K. I., Garcia R. O., Gerth K., Irschik H., Müller R. ( 2012 ). Sandaracinus amylolyticus gen. nov., sp. nov., a starch-degrading soil myxobacterium, and description of Sandaracinaceae fam. nov.. Int J Syst Evol Microbiol 62, 1191–1198. [View Article] [PubMed]
    [Google Scholar]
  25. Morgulis A., Coulouris G., Raytselis Y., Madden T. L., Agarwala R., Schäffer A. A. ( 2008 ). Database indexing for production MegaBLAST searches. . Bioinformatics 24, 1757–1764. [View Article] [PubMed]
    [Google Scholar]
  26. Nichols D. S., McMeekin T. A. ( 2002 ). Biomarker techniques to screen for bacteria that produce polyunsaturated fatty acids. . J Microbiol Methods 48, 161–170. [View Article] [PubMed]
    [Google Scholar]
  27. Nichols D., Bowman J., Sanderson K., Nichols C. M., Lewis T., McMeekin T., Nichols P. D. ( 1999 ). Developments with Antarctic microorganisms: culture collections, bioactivity screening, taxonomy, PUFA production and cold-adapted enzymes. . Curr Opin Biotechnol 10, 240–246. [View Article] [PubMed]
    [Google Scholar]
  28. Ohlendorf B., Kehraus S., König G. M. ( 2008 ). Myxochromide B3, a new member of the myxochromide family of secondary metabolites. . J Nat Prod 71, 1708–1713. [View Article] [PubMed]
    [Google Scholar]
  29. Plaza A., Garcia R., Bifulco G., Martinez J. P., Hüttel S., Sasse F., Meyerhans A., Stadler M., Müller R. ( 2012 ). Aetheramides A and B, potent HIV-inhibitory depsipeptides from a myxobacterium of the new genus “Aetherobacter”. . Org Lett 14, 2854–2857. [View Article] [PubMed]
    [Google Scholar]
  30. Plaza A., Viehrig K., Garcia R., Müller R. ( 2013 ). Jahnellamides, α-keto-β-methionine-containing peptides from the terrestrial myxobacterium Jahnella sp.: structure and biosynthesis. . Org Lett 15, 5882–5885. [View Article] [PubMed]
    [Google Scholar]
  31. Reichenbach H. ( 2005 ). Order VIII. Myxococcales Tchan, Pochon and Prévot 1948, 398AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, pp. 1059–1072. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
     [Google Scholar]
  32. Reichenbach H., Dworkin M. ( 1992 ). The myxobacteria. . In The Prokaryotes, , 2nd edn., pp. 3416–3487. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. . Berlin:: Springer;. [View Article]
    [Google Scholar]
  33. Reichenbach H., Kleinig H. ( 1971 ). The carotenoids of Myxococcus fulvus (Myxobacterales). . Arch Mikrobiol 76, 364–380. [View Article]
    [Google Scholar]
  34. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  35. Sanford R. A., Cole J. R., Tiedje J. M. ( 2002 ). Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an aryl-halorespiring facultative anaerobic myxobacterium. . Appl Environ Microbiol 68, 893–900. [View Article] [PubMed]
    [Google Scholar]
  36. Shimelis O., Giese R. W. ( 2006 ). Nuclease P1 digestion/high-performance liquid chromatography, a practical method for DNA quantitation. . J Chromatogr A 1117, 132–136. [View Article] [PubMed]
    [Google Scholar]
  37. Shimkets L. J., Dworkin M., Reichenbach H. ( 2006 ). The myxobacteria. . In The Prokaryotes, , 3rd edn., vol. 7, pp. 31–115. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  38. Sood S., Awal R. P., Wink J., Mohr K. I., Rohde M., Stadler M., Kämpfer P., Glaeser S., Schumann P. & other authors ( 2014 ). Aggregicoccus edonensis gen. nov., sp. nov., an unusually aggregating myxobacterium isolated from a soil sample. . Int J Syst Evol Microbiol (in press). [View Article] [PubMed]
    [Google Scholar]
  39. Stadler M., Roemer E., Müller R., Garcia R. O., Pistorius D., Brachmann A. ( 2010 ). Production of omega-3 fatty acids by myxobacteria. International patent WO 2010/063451 A2.
     [Google Scholar]
  40. Trowitzsch Kienast W., Gerth K., Reichenbach H., Höfle G. ( 1993 ). Myxochromid A: ein hochungesättigtes Lipopeptidlacton aus Myxococcus virescens . . Liebigs Ann Chem 1993, 1233–1237 (in German). [View Article]
    [Google Scholar]
  41. Weissman K. J., Müller R. ( 2010 ). Myxobacterial secondary metabolites: bioactivities and modes-of-action. . Nat Prod Rep 27, 1276–1295. [View Article] [PubMed]
    [Google Scholar]
  42. Zeggel B. ( 1993 ). Steroide bei Myxobakterien. Thesis, Technische Universität Carolo-Wilhelmina zu Braunschweig;, Braunschweig, Germany (in German):.
     [Google Scholar]
  43. Zhang Z., Schwartz S., Wagner L., Miller W. ( 2000 ). A greedy algorithm for aligning DNA sequences. . J Comput Biol 7, 203–214. [View Article] [PubMed]
    [Google Scholar]
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Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium
Int J Syst Evol Microbiol 64, 3733 (2014); https://doi.org/10.1099/ijs.0.068270-0
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