1887

Abstract

A Gram-staining-negative, non-motile, catalase- and oxidase-positive strain, designated CCNWSP36-1, was isolated from the nodule surface of soybean [ (L.) Merrill] cultivar Zhonghuang 13. The 16S rRNA gene sequence analysis clearly showed that the isolate represented a member of the genus . On the basis of pairwise comparisons of 16S rRNA gene sequences, strain CCNWSP36-1 showed 96.8 % similarity to CCTCC AB 2010390 and less than 95.2 % similarity to other members of the genus . Growth of strain CCNWSP36-1 occurred at 10–40 °C and at pH 5.0–9.0. The NaCl range (w/v) for growth was 0–4 %. The predominant isoprenoid quinone was MK-7. The polar lipids were phosphatidylethanolamine and several unidentified polar lipids. Sphingolipid was present. The major fatty acids were iso-C and summed feature 3 (comprising Cω6 and/or Cω7). The G+C content of the genomic DNA was 41.1 mol%. As the physiological and biochemical characteristics of strain CCNWSP36-1 and the type strains of its closest phylogenetic neighbours showed clear differences, a novel species, , is proposed. The type strain is CCNWSP36-1 ( = ACCC 19328 = JCM 30166).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.068254-0
2014-11-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3862.html?itemId=/content/journal/ijsem/10.1099/ijs.0.068254-0&mimeType=html&fmt=ahah

References

  1. Albert R. A., Waas N. E., Pavlons S. C., Pearson J. L., Ketelboeter L., Rosselló-Móra R., Busse H. J.. ( 2013;). Sphingobacterium psychroaquaticum sp. nov., a psychrophilic bacterium isolated from Lake Michigan water. . Int J Syst Evol Microbiol 63:, 952–958. [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Blackwood C. B., Hudleston D., Zak D. R., Buyer J. S.. ( 2007;). Interpreting ecological diversity indices applied to terminal restriction fragment length polymorphism data: insights from simulated microbial communities. . Appl Environ Microbiol 73:, 5276–5283. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Microbiol 48:, 459–470.
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  6. Duan S., Liu Z., Feng X., Zheng K., Cheng L.. ( 2009;). Sphingobacterium bambusae sp. nov., isolated from soil of bamboo plantation. . J Microbiol 47:, 693–698. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A.. ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef][PubMed]
    [Google Scholar]
  10. Khan S. T., Takaichi S., Harayama S.. ( 2008;). Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. . Int J Syst Evol Microbiol 58:, 383–386. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Liu J., Yang L. L., Xu C. K., Xi J. Q., Yang F. X., Zhou F., Zhou Y., Mo M. H., Li W. J.. ( 2012;). Sphingobacterium nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco. . Int J Syst Evol Microbiol 62:, 1809–1813. [CrossRef][PubMed]
    [Google Scholar]
  13. Matsuyama H., Katoh H., Ohkushi T., Satoh A., Kawahara K., Yumoto I.. ( 2008;). Sphingobacterium kitahiroshimense sp. nov., isolated from soil. . Int J Syst Evol Microbiol 58:, 1576–1579. [CrossRef][PubMed]
    [Google Scholar]
  14. Mehnaz S., Weselowski B., Lazarovits G.. ( 2007;). Sphingobacterium canadense sp. nov., an isolate from corn roots. . Syst Appl Microbiol 30:, 519–524. [CrossRef][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Schmidt V. S., Wenning M., Scherer S.. ( 2012;). Sphingobacterium lactis sp. nov. and Sphingobacterium alimentarium sp. nov., isolated from raw milk and a dairy environment. . Int J Syst Evol Microbiol 62:, 1506–1511. [CrossRef][PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J.. ( 1998;). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48:, 165–177. [CrossRef][PubMed]
    [Google Scholar]
  21. Sun L. N., Zhang J., Chen Q., He J., Li S. P.. ( 2013;). Sphingobacterium caeni sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 63:, 2260–2264. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  23. Wei W., Zhou Y., Wang X., Huang X., Lai R.. ( 2008;). Sphingobacterium anhuiense sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 58:, 2098–2101. [CrossRef][PubMed]
    [Google Scholar]
  24. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N.. ( 1983;). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting Gram-negative rods in CDC groups IIK-2 and IIb. . Int J Syst Bacteriol 33:, 580–598. [CrossRef]
    [Google Scholar]
  25. Yoo S. H., Weon H. Y., Jang H. B., Kim B. Y., Kwon S. W., Go S. J., Stackebrandt E.. ( 2007;). Sphingobacterium composti sp. nov., isolated from cotton-waste composts. . Int J Syst Evol Microbiol 57:, 1590–1593. [CrossRef][PubMed]
    [Google Scholar]
  26. Zhao L., Deng Z., Yang W., Cao Y., Wang E., Wei G.. ( 2010;). Diverse rhizobia associated with Sophora alopecuroides grown in different regions of Loess Plateau in China. . Syst Appl Microbiol 33:, 468–477. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.068254-0
Loading
/content/journal/ijsem/10.1099/ijs.0.068254-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error