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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_11

sp. nov., isolated from the nodule surface of soybean Free

Abstract

A Gram-staining-negative, non-motile, catalase- and oxidase-positive strain, designated CCNWSP36-1, was isolated from the nodule surface of soybean [ (L.) Merrill] cultivar Zhonghuang 13. The 16S rRNA gene sequence analysis clearly showed that the isolate represented a member of the genus . On the basis of pairwise comparisons of 16S rRNA gene sequences, strain CCNWSP36-1 showed 96.8 % similarity to CCTCC AB 2010390 and less than 95.2 % similarity to other members of the genus . Growth of strain CCNWSP36-1 occurred at 10–40 °C and at pH 5.0–9.0. The NaCl range (w/v) for growth was 0–4 %. The predominant isoprenoid quinone was MK-7. The polar lipids were phosphatidylethanolamine and several unidentified polar lipids. Sphingolipid was present. The major fatty acids were iso-C and summed feature 3 (comprising Cω6 and/or Cω7). The G+C content of the genomic DNA was 41.1 mol%. As the physiological and biochemical characteristics of strain CCNWSP36-1 and the type strains of its closest phylogenetic neighbours showed clear differences, a novel species, , is proposed. The type strain is CCNWSP36-1 ( = ACCC 19328 = JCM 30166).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31000943 and 31125007)
© 2014 IUMS
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2014-11-01
2025-04-23
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Sphingobacteriumyanglingense sp. nov., isolated from the nodule surface of soybean
Int J Syst Evol Microbiol 64, 3862 (2014); https://doi.org/10.1099/ijs.0.068254-0
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