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Volume 65, Issue Pt_2

gen. nov., sp. nov., a piezophilic, thermophilic, anaerobic, fermentative bacterium isolated from a deep-sea hydrothermal vent Free

Abstract

A novel piezophilic, thermophilic, anaerobic, fermentative bacterial strain, designated strain DY22613, was isolated from a deep-sea hydrothermal sulfide deposit at the East Pacific Rise (GPS position: 102.6° W 3.1° S). Cells of strain DY22613 were long, motile rods (10 to 20 µm in length and 0.5 µm in width) with peritrichous flagella and were Gram-stain-negative. Growth was recorded at 44–72 °C (optimum 60–62 °C) and at hydrostatic pressures of 0.1–55 MPa (optimum 20 MPa). The pH range for growth was from pH 5.0 to 9.0 with an optimum at pH 7.0. Growth was observed in the presence of 1 to 8 % (w/v) sea salts and 0.65 to 5.2 % (w/v) NaCl, with optimum salt concentrations at 3.5 % for sea salts and at 2.3 % for NaCl. Under optimal growth conditions, the shortest generation time observed was 27 min (60 °C, 20 MPa). Strain DY22613 was heterotrophic, able to utilize complex organic compounds, amino acids, sugars and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamine, methionine, phenylalanine, serine, threonine, fructose, fucose, galactose, gentiobiose, glucose, mannose, melibiose, palatinose, rhamnose, turanose, pyruvate, lactic acid, methyl ester, erythritol, galacturonic acid and glucosaminic acid. Strain DY22613 was able to reduce Fe(III) compounds, including Fe(III) oxyhydroxide (pH 7.0), amorphous iron(III) oxide (pH 9.0), goethite (α-FeOOH, pH 12.0), Fe(III) citrate and elementary sulfur. Products of fermentation were butyrate, acetate and hydrogen. Main cellular fatty acids were iso-C, iso-C 3-OH and C. The genomic DNA G+C content of strain DY22613 was 36.7 mol%. Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class and clusters with the order (86.92 % 16S rRNA gene sequence similarity). The phylogenetic data suggest that the lineage represents at least a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is DY22613 ( = JCM 19466 = DSM 28033 = MCCC 1A06456).

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Funding
This study was supported by the:
  • National Program on Key Basic Research Project (Award 2012CB417304)
  • the National High Technology Research and Development Program of China (Award 2012AA092103)
  • National Infrastructure of Microbial Resources (Award NIRM2014-9)
  • MaCuMBA (Award 311975)
  • PICS-CNRS-INEE Phypress
  • PHC Cai YuanPei (Award 30412WG)
  • PHC Cai YuanPei (Award 30412WG)
  • PHC Cai YuanPei (Award 30412WG)
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2015-02-01
2024-09-14
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References

  1. Alain K., Pignet P., Zbinden M., Quillevere M., Duchiron F., Donval J. P., Lesongeur F., Raguenes G., Crassous P. & other authors ( 2002 ). Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. . Int J Syst Evol Microbiol 52, 1621–1628. [View Article] [PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 3389–3402. [View Article] [PubMed]
    [Google Scholar]
  3. Ben Aissa F., Postec A., Erauso G., Payri C., Pelletier B., Hamdi M., Ollivier B., Fardeau M. L. ( 2014 ). Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney. . Int J Syst Evol Microbiol 64, 1160–1165. [View Article] [PubMed]
    [Google Scholar]
  4. Brisbarre N., Fardeau M. L., Cueff V., Cayol J. L., Barbier G., Cilia V., Ravot G., Thomas P., Garcia J. L., Ollivier B. ( 2003 ). Clostridium caminithermale sp. nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney. . Int J Syst Evol Microbiol 53, 1043–1049. [View Article] [PubMed]
    [Google Scholar]
  5. Cayol J. L., Ollivier B., Patel B. K., Prensier G., Guezennec J., Garcia J. L. ( 1994 ). Isolation and characterization of Halothermothrix orenii gen. nov., sp. nov., a halophilic, thermophilic, fermentative, strictly anaerobic bacterium. . Int J Syst Bacteriol 44, 534–540. [View Article] [PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 2259–2261. [View Article] [PubMed]
    [Google Scholar]
  7. Fardeau M. L., Bonilla Salinas M., L’Haridon S., Jeanthon C., Verhé F., Cayol J. L., Patel B. K., Garcia J. L., Ollivier B. ( 2004 ). Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies. . Int J Syst Evol Microbiol 54, 467–474. [View Article] [PubMed]
    [Google Scholar]
  8. Hogan C. M. ( 2010 ). Extremophile. . In Encyclopedia of Earth. Edited by Monosson E., Cleveland C. . Washington, DC:: National Council for Science and the Environment;.
     [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  10. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. . E. Stackebrandt & M. Goodfellow, eds. New York, NY:, John Wiley and Sons;: 115–175.
     [Google Scholar]
  11. Lovley D. R., Phillips E. J. P. ( 1986a ). Availability of ferric iron for microbial reduction in bottom sediments of the freshwater tidal potomac river. . Appl Environ Microbiol 52, 751–757.[PubMed]
    [Google Scholar]
  12. Lovley D. R., Phillips E. J. P. ( 1986b ). Organic matter mineralization with reduction of ferric iron in anaerobic sediments. . Appl Environ Microbiol 51, 683–689.[PubMed]
    [Google Scholar]
  13. Mesbah N. M., Wiegel J. ( 2009 ). Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt. . Int J Syst Evol Microbiol 59, 2042–2048. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah N. M., Hedrick D. B., Peacock A. D., Rohde M., Wiegel J. ( 2007 ). Natranaerobius thermophilus gen. nov., sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt, and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov.. Int J Syst Evol Microbiol 57, 2507–2512. [View Article] [PubMed]
    [Google Scholar]
  15. Miroshnichenko M. L., Bonch-Osmolovskaya E. A. ( 2006 ). Recent developments in the thermophilic microbiology of deep-sea hydrothermal vents. . Extremophiles 10, 85–96. [View Article] [PubMed]
    [Google Scholar]
  16. Nepomnyashchaya Y. N., Slobodkina G. B., Baslerov R. V., Chernyh N. A., Bonch-Osmolovskaya E. A., Netrusov A. I., Slobodkin A. I. ( 2012 ). Moorella humiferrea sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III). . Int J Syst Evol Microbiol 62, 613–617. [View Article] [PubMed]
    [Google Scholar]
  17. Rainey F. A. ( 2009 ). Class II. Clostridia . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 736. Edited by De vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . Dordrecht:: Springer;.
     [Google Scholar]
  18. Rainey F. A., Zhilina T. N., Boulygina E. S., Stackebrandt E., Tourova T. P., Zavarzin G. A. ( 1995 ). The taxonomic status of the fermentative halophilic anaerobic bacteria: description of Haloanaerobiales ord. nov., Halobacteroidaceae fam. nov., Orenia gen. nov. and further taxonomic rearrangements at the genus and species level. . Anaerobe 1, 185–199. [View Article] [PubMed]
    [Google Scholar]
  19. Reynolds E. S. ( 1963 ). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. . J Cell Biol 17, 208–212. [View Article] [PubMed]
    [Google Scholar]
  20. Slobodkin A. I., Tourova T. P., Kostrikina N. A., Chernyh N. A., Bonch-Osmolovskaya E. A., Jeanthon C., Jones B. E. ( 2003 ). Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 53, 1131–1134. [View Article] [PubMed]
    [Google Scholar]
  21. Slobodkina G. B., Kolganova T. V., Tourova T. P., Kostrikina N. A., Jeanthon C., Bonch-Osmolovskaya E. A., Slobodkin A. I. ( 2008 ). Clostridium tepidiprofundi sp. nov., a moderately thermophilic bacterium from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 58, 852–855. [View Article] [PubMed]
    [Google Scholar]
  22. Urios L., Cueff V., Pignet P., Barbier G. ( 2004 ). Tepidibacter formicigenes sp. nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent. . Int J Syst Evol Microbiol 54, 439–443. [View Article] [PubMed]
    [Google Scholar]
  23. Wery N., Moricet J. M., Cueff V., Jean J., Pignet P., Lesongeur F., Cambon-Bonavita M. A., Barbier G. ( 2001 ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 51, 1789–1796. [View Article] [PubMed]
    [Google Scholar]
  24. Yarza P., Yilmaz P., Pruesse E., Glöckner F. O., Ludwig W., Schleifer K.-H., Whitman W. B., Euzéby J., Amann R., Rosselló-Móra R. ( 2014 ). Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. . Nat Rev Microbiol 12, 635–645. [View Article] [PubMed]
    [Google Scholar]
  25. Zhang Z., Schwartz S., Wagner L., Miller W. ( 2000 ). A greedy algorithm for aligning DNA sequences. . J Comput Biol 7, 203–214. [View Article] [PubMed]
    [Google Scholar]
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Anoxybacter fermentans gen. nov., sp. nov., a piezophilic, thermophilic, anaerobic, fermentative bacterium isolated from a deep-sea hydrothermal vent
Int J Syst Evol Microbiol 65, 710 (2015); https://doi.org/10.1099/ijs.0.068221-0
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