1887

Abstract

A Gram-stain-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, rod-shaped bacterium, strain MY11, was isolated from a sediment sample collected from the Western Pacific. This isolate grew in the presence of 0.5–18 % (w/v) NaCl and at pH 6.0–10.0 and 15–45 °C; optimum growth was observed with 3.5 % (w/v) NaCl and at pH 8.0–9.0 and 35–37 °C. Strain MY11 had menaquinone 7 (MK-7) as the predominant respiratory quinone and anteiso-C and anteiso-C as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 34.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain MY11 was a member of the genus , exhibiting sequence similarities of 95.3–97.6 % to the type strains of recognized species. Strain MY11 could be differentiated from recognized species of the genus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of the data presented, strain MY11 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MY11 ( = LMG 28105 = CGMCC 1.12754 = MCCC 1A09973).

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2015-01-01
2019-11-14
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References

  1. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  2. Carrasco I. J., Márquez M. C., Ventosa A.. ( 2009;). Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake. . Int J Syst Evol Microbiol 59:, 3068–3073. [CrossRef][PubMed]
    [Google Scholar]
  3. Chen Y. G., Cui X. L., Fritze D., Chai L. H., Schumann P., Wen M. L., Wang Y. X., Xu L. H., Jiang C. L.. ( 2008;). Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 58:, 647–653. [CrossRef][PubMed]
    [Google Scholar]
  4. Chen Y. G., Liu Z. X., Peng D. J., Zhang Y. Q., Wang Y. X., Tang S. K., Li W. J., Cui X. L., Liu Y. Q.. ( 2009;). Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil. . Antonie van Leeuwenhoek 96:, 323–329. [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Doetsch R.. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Gregersen T.. ( 1978;). Rapid method for distinction of gram-negative from gram-positive bacteria. . Eur J Appl Microbiol Biot 5:, 123–127. [CrossRef]
    [Google Scholar]
  9. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A.. ( 1998;). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. . Int J Syst Bacteriol 48:, 99–106. [CrossRef]
    [Google Scholar]
  10. Heyndrickx M., Lebbe L., Kersters K., Hoste B., De Wachter R., De Vos P., Forsyth G., Logan N. A.. ( 1999;). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. . Int J Syst Bacteriol 49:, 1083–1090. [CrossRef][PubMed]
    [Google Scholar]
  11. Heyrman J., Logan N. A., Busse H. J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P.. ( 2003;). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. . Int J Syst Evol Microbiol 53:, 501–511. [CrossRef][PubMed]
    [Google Scholar]
  12. Huss V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  13. Jeon C. O., Kim J. M., Park D. J., Xu L. H., Jiang C. L., Kim C. J.. ( 2009;). Virgibacillus xinjiangensis sp. nov., isolated from a Salt Lake of Xin-jiang Province in China. . J Microbiol 47:, 705–709. [CrossRef][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  19. Nei M., Kumar S.. ( 2000;). Molecular Evolution and Phylogenetics. New York:: Oxford University Press;.
    [Google Scholar]
  20. Niederberger T. D., Steven B., Charvet S., Barbier B., Whyte L. G.. ( 2009;). Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic. . Int J Syst Evol Microbiol 59:, 2219–2225. [CrossRef][PubMed]
    [Google Scholar]
  21. Quesada T., Aguilera M., Morillo J. A., Ramos-Cormenzana A., Monteoliva-Sánchez M.. ( 2007;). Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives. . Int J Syst Evol Microbiol 57:, 906–910. [CrossRef][PubMed]
    [Google Scholar]
  22. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  25. Yoon J. H., Oh T. K., Park Y. H.. ( 2004;). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov.. Int J Syst Evol Microbiol 54:, 2163–2167. [CrossRef][PubMed]
    [Google Scholar]
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