1887

Abstract

A Gram-stain-variable, rod-shaped and endospore-forming bacterium, designated strain L7-75, was isolated from duckweed (). Cells were motile with a monopolar flagellum. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L7-75 belonged to the genus , and the closest phylogenetically related species were N3/975 (98.5 % 16S rRNA gene sequence similarity), ES_M17 (98.5 %), MB 1871 (98.2 %), 324 (97.7 %), S93 (97.7 %) and ATCC 43898 (97.4 %). Growth of strain L7-75 was observed at pH 7–10 and at 20–40 °C, and NaCl concentrations up to 5 % (w/v) were tolerated. Major cellular fatty acids included anteiso-C, C and anteiso-C that were present at 36.0 %, 14.2 % and 10.0 % of the total cellular fatty acid profile, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl--methylethanolamine. MK-7 was the predominant menaquinone. The diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. The DNA G+C content was 49.1 mol% ( ). DNA–DNA relatedness values between strain L7-75 and its closest relatives ranged from 4.4 to 47.8 %. These results indicate that strain L7-75 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L7-75 ( = BCC 67838 = NBRC 109972).

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2015-01-01
2024-03-28
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References

  1. Araújo W. L., Marcon J., Maccheroni W. Jr, Van Elsas J. D., Van Vuurde J. W., Azevedo J. L. ( 2002 ). Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. . Appl Environ Microbiol 68, 49064914. [View Article] [PubMed]
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. ( 1993 ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. . Antonie van Leeuwenhoek 64, 253260. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  4. Dsouza M., Taylor M. W., Ryan J., MacKenzie A., Lagutin K., Anderson R. F., Turner S. J., Aislabie J. ( 2014 ). Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil. . Int J Syst Evol Microbiol 64, 14061411. [View Article] [PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  9. Glaeser S. P., Falsen E., Busse H. J., Kämpfer P. ( 2013 ). Paenibacillus vulneris sp. nov., isolated from a necrotic wound. . Int J Syst Evol Microbiol 63, 777782. [View Article] [PubMed]
    [Google Scholar]
  10. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1, 138146. [View Article]
    [Google Scholar]
  11. Graham P. H., Parker C. A. ( 1964 ). Diagnostic features in the characterization of the root-nodule bacteria of legumes. . Plant Soil 20, 383396. [View Article]
    [Google Scholar]
  12. Jin H. J., Zhou Y. G., Liu H. C., Chen S. F. ( 2011 ). Paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Begonia semperflorens . . Int J Syst Evol Microbiol 61, 13501355. [View Article] [PubMed]
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  14. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H. J., Tindall B. J. ( 2006 ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56, 781786. [View Article] [PubMed]
    [Google Scholar]
  15. Kawamoto I., Oka T., Nara T. ( 1981 ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146, 527534.[PubMed]
    [Google Scholar]
  16. Kim H. S., Lee S. S. ( 2014 ). Paenibacillus humi sp. nov., isolated from peat-soil. . J Gen Appl Microbiol 60, 2327. [View Article] [PubMed]
    [Google Scholar]
  17. Kim B. C., Lee K. H., Kim M. N., Kim E. M., Min S. R., Kim H. S., Shin K. S. ( 2009 ). Paenibacillus pini sp. nov., a cellulolytic bacterium isolated from the rhizosphere of pine tree. . J Microbiol 47, 699704. [View Article] [PubMed]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  19. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  20. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic acid techniques in bacterial systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley & Sons;.
    [Google Scholar]
  21. Lim J. M., Jeon C. O., Park D. J., Xu L. H., Jiang C. L., Kim C. J. ( 2006 ). Paenibacillus xinjiangensis sp. nov., isolated from Xinjiang province in China. . Int J Syst Evol Microbiol 56, 25792582. [View Article] [PubMed]
    [Google Scholar]
  22. Liu Y., Liu L., Qiu F., Schumann P., Shi Y., Zou Y., Zhang X., Song W. ( 2010 ). Paenibacillus hunanensis sp. nov., isolated from rice seeds. . Int J Syst Evol Microbiol 60, 12661270. [View Article] [PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  25. Moore D. D., Dowhan D. ( 1995 ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 211. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Wiley;.
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  27. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acid . , MIDI Technical Note 101. Newark, DE: MIDI Inc.
    [Google Scholar]
  28. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. ( 1997 ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . . Int J Syst Bacteriol 47, 289298. [View Article] [PubMed]
    [Google Scholar]
  29. Skerman V. B. D. ( 1967 ). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  30. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33, 152155.
    [Google Scholar]
  32. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  34. Tang Q. Y., Yang N., Wang J., Xie Y. Q., Ren B., Zhou Y. G., Gu M. Y., Mao J., Li W. J. et al. ( 2011 ). Paenibacillus algorifonticola sp. nov., isolated from a cold spring. . Int J Syst Evol Microbiol 61, 21672172. [View Article] [PubMed]
    [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  36. Valverde A., Peix A., Rivas R., Velázquez E., Salazar S., Santa-Regina I., Rodríguez-Barrueco C., Igual J. M. ( 2008 ). Paenibacillus castaneae sp. nov., isolated from the phyllosphere of Castanea sativa Miller. . Int J Syst Evol Microbiol 58, 25602564. [View Article] [PubMed]
    [Google Scholar]
  37. Valverde A., Fterich A., Mahdhi M., Ramírez-Bahena M. H., Caviedes M. A., Mars M., Velázquez E., Rodriguez-Llorente I. D. ( 2010 ). Paenibacillus prosopidis sp. nov., isolated from the nodules of Prosopis farcta . . Int J Syst Evol Microbiol 60, 21822186. [View Article] [PubMed]
    [Google Scholar]
  38. Vaz-Moreira I., Figueira V., Lopes A. R., Pukall R., Spröer C., Schumann P., Nunes O. C., Manaia C. M. ( 2010 ). Paenibacillus residui sp. nov., isolated from urban waste compost. . Int J Syst Evol Microbiol 60, 24152419. [View Article] [PubMed]
    [Google Scholar]
  39. Verlander C. P. ( 1992 ). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185201. Edited by Kricka L. J. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  40. Wang L., Baek S. H., Cui Y., Lee H. G., Lee S. T. ( 2012 ). Paenibacillus sediminis sp. nov., a xylanolytic bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 62, 12841288. [View Article] [PubMed]
    [Google Scholar]
  41. Wang L. Y., Li J., Li Q. X., Chen S. F. ( 2013 ). Paenibacillus beijingensis sp. nov., a nitrogen-fixing species isolated from wheat rhizosphere soil. . Antonie van Leeuwenhoek 104, 675683. [View Article] [PubMed]
    [Google Scholar]
  42. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  43. Wu X., Fang H., Qian C., Wen Y., Shen X., Li O., Gao H. ( 2011 ). Paenibacillus tianmuensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61, 11331137. [View Article] [PubMed]
    [Google Scholar]
  44. Wu Y.-F., Wu Q.-L., Liu S.-J. ( 2013 ). Paenibacillus taihuensis sp. nov., isolated from an eutrophic lake. . Int J Syst Evol Microbiol 63, 36523658. [View Article] [PubMed]
    [Google Scholar]
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