1887

Abstract

A Gram-stain-variable, rod-shaped and endospore-forming bacterium, designated strain L7-75, was isolated from duckweed (). Cells were motile with a monopolar flagellum. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L7-75 belonged to the genus , and the closest phylogenetically related species were N3/975 (98.5 % 16S rRNA gene sequence similarity), ES_M17 (98.5 %), MB 1871 (98.2 %), 324 (97.7 %), S93 (97.7 %) and ATCC 43898 (97.4 %). Growth of strain L7-75 was observed at pH 7–10 and at 20–40 °C, and NaCl concentrations up to 5 % (w/v) were tolerated. Major cellular fatty acids included anteiso-C, C and anteiso-C that were present at 36.0 %, 14.2 % and 10.0 % of the total cellular fatty acid profile, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl--methylethanolamine. MK-7 was the predominant menaquinone. The diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. The DNA G+C content was 49.1 mol% ( ). DNA–DNA relatedness values between strain L7-75 and its closest relatives ranged from 4.4 to 47.8 %. These results indicate that strain L7-75 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L7-75 ( = BCC 67838 = NBRC 109972).

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2015-01-01
2019-11-21
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