1887

Abstract

A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7, isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus , showing more than 97.0 % sequence similarity with RU-04 (98.0 %), RU-16 (97.7 %) and PU-02 (97.3 %). However, DNA–DNA relatedness values demonstrated that strain KIS12-7 could be clearly distinguished from closely related species of the genus . The cell-wall peptidoglycan of strain KIS12-7 was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7 is a representative of a novel species of the genus , and the name sp. nov. is proposed; the type strain is KIS12-7 ( = DSM 27182 = KACC 17302 = NBRC 109659).

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2014-12-01
2019-11-20
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