1887

Abstract

A novel Gram-stain-positive, non-motile, facultatively anaerobic and rod-shaped bacterium, strain PSPT56, was isolated from the gastrointestinal tract of a pen shell (). Optimal growth of strain PSPT56 was ascertained to occur at 30 °C, pH 8.0 and in the presence of 1–2 % (w/v) NaCl. The strain was catalase-positive and oxidase-negative. The major cellular fatty acids were Cω9, C, Cω8 and C. Tuberculostearic acid was not present. The major cell-wall sugars were ribose, galactose, glucose and arabinose. Peptidoglycan amino acids were -diaminopimelic acid, alanine and glutamic acid. The predominant isoprenoid quinone was MK-8(H). Strain PSPT56 contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified lipids and two unidentified amino-lipids. Mycolic acids were detected as constitutive components of the cell wall. A phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain PSPT56 was most closely related to M935/96/4 and M714/95/5 with 98.69 % and 97.01 % similarity, respectively. DNA–DNA hybridization experiments indicated less than 29.9 % relatedness to the phylogenetically closest species. The G+C content of genomic DNA was 67.6 mol%. The phenotypic, phylogenetic and genotypic analyses indicated that strain PSPT56 represents a novel species within the genus , for which the name is proposed. The type strain is PSPT56 ( = KACC 17525 = JCM 19266).

Funding
This study was supported by the:
  • National Research Foundation (Award 2012-0008806)
  • National Institute of Biological Resources (Award 2013-02-001)
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2015-02-01
2021-04-17
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