1887

Abstract

A novel Gram-stain-positive, non-motile, facultatively anaerobic and rod-shaped bacterium, strain PSPT56, was isolated from the gastrointestinal tract of a pen shell (). Optimal growth of strain PSPT56 was ascertained to occur at 30 °C, pH 8.0 and in the presence of 1–2 % (w/v) NaCl. The strain was catalase-positive and oxidase-negative. The major cellular fatty acids were Cω9, C, Cω8 and C. Tuberculostearic acid was not present. The major cell-wall sugars were ribose, galactose, glucose and arabinose. Peptidoglycan amino acids were -diaminopimelic acid, alanine and glutamic acid. The predominant isoprenoid quinone was MK-8(H). Strain PSPT56 contained phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified lipids and two unidentified amino-lipids. Mycolic acids were detected as constitutive components of the cell wall. A phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain PSPT56 was most closely related to M935/96/4 and M714/95/5 with 98.69 % and 97.01 % similarity, respectively. DNA–DNA hybridization experiments indicated less than 29.9 % relatedness to the phylogenetically closest species. The G+C content of genomic DNA was 67.6 mol%. The phenotypic, phylogenetic and genotypic analyses indicated that strain PSPT56 represents a novel species within the genus , for which the name is proposed. The type strain is PSPT56 ( = KACC 17525 = JCM 19266).

Funding
This study was supported by the:
  • National Research Foundation (Award 2012-0008806)
  • National Institute of Biological Resources (Award 2013-02-001)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.067587-0
2015-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/2/531.html?itemId=/content/journal/ijsem/10.1099/ijs.0.067587-0&mimeType=html&fmt=ahah

References

  1. Bae J. W., Rhee S. K., Park J. R., Chung W. H., Nam Y. D., Lee I., Kim H., Park Y. H. ( 2005 ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. . Appl Environ Microbiol 71, 88258835. [View Article] [PubMed]
    [Google Scholar]
  2. Bernard K. A., Funke G. ( 2012 ). Genus Corynebacterium.. In Bergey’s Manual of Systematic Bacteriology, 2nd edn, vol. 5, The Actinobacteria pp. 245289. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  3. Bousfield G. R., Sugino H., Ward D. N. ( 1985 ). Demonstration of a COOH-terminal extension on equine lutropin by means of a common acid-labile bond in equine lutropin and equine chorionic gonadotropin. . J Biol Chem 260, 95319533.[PubMed]
    [Google Scholar]
  4. Brennan N. M., Brown R., Goodfellow M., Ward A. C., Beresford T. P., Simpson P. J., Fox P. F., Cogan T. M. ( 2001 ). Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. . Int J Syst Evol Microbiol 51, 843852. [View Article] [PubMed]
    [Google Scholar]
  5. Chang H. W., Nam Y. D., Jung M. Y., Kim K. H., Roh S. W., Kim M. S., Jeon C. O., Yoon J. H., Bae J. W. ( 2008 ). Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. . J Microbiol Methods 75, 523530. [View Article] [PubMed]
    [Google Scholar]
  6. Collins M. D., Jones D. ( 1981 ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316354.[PubMed]
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  8. Collins M. D., Goodfellow M., Minnikin D. E. ( 1982 ). A survey of the structures of mycolic acids in Corynebacterium and related taxa. . J Gen Microbiol 128, 129149.[PubMed]
    [Google Scholar]
  9. Collins M. D., Goodfellow M., Minnikin D. E., Alderson G. ( 1985 ). Menaquinone composition of mycolic acid-containing actinomycetes and some sporoactinomycetes. . J Appl Bacteriol 58, 7786. [View Article] [PubMed]
    [Google Scholar]
  10. Collins M. D., Hoyles L., Hutson R. A., Foster G., Falsen E. ( 2001 ). Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. . Int J Syst Evol Microbiol 51, 13491352.[PubMed]
    [Google Scholar]
  11. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  12. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  13. Feurer C., Clermont D., Bimet F., Candréa A., Jackson M., Glaser P., Bizet C., Dauga C. ( 2004 ). Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.. Int J Syst Evol Microbiol 54, 10551061. [View Article] [PubMed]
    [Google Scholar]
  14. Frischmann A., Knoll A., Hilbert F., Zasada A. A., Kämpfer P., Busse H. J. ( 2012 ). Corynebacterium epidermidicanis sp. nov., isolated from skin of a dog. . Int J Syst Evol Microbiol 62, 21942200. [View Article] [PubMed]
    [Google Scholar]
  15. Funke G., Hutson R. A., Hilleringmann M., Heizmann W. R., Collins M. D. ( 1997 ). Corynebacterium lipophiloflavum sp. nov. isolated from a patient with bacterial vaginosis. . FEMS Microbiol Lett 150, 219224. [View Article] [PubMed]
    [Google Scholar]
  16. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  17. Goodfellow M., Collins M. D., Minnikin D. E. ( 1976 ). Thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa. . J Gen Microbiol 96, 351358. [View Article] [PubMed]
    [Google Scholar]
  18. Goyache J., Vela A. I., Collins M. D., Ballesteros C., Briones V., Moreno J., Yorio P., Domínguez L., Hutson R., Fernández-Garayzábal J. F. ( 2003a ). Corynebacterium spheniscorum sp. nov., isolated from the cloacae of wild penguins. . Int J Syst Evol Microbiol 53, 4346. [View Article] [PubMed]
    [Google Scholar]
  19. Goyache J., Ballesteros C., Vela A. I., Collins M. D., Briones V., Hutson R. A., Potti J., García-Borboroglu P., Domínguez L., Fernández-Garayzábal J. F. ( 2003b ). Corynebacterium sphenisci sp. nov., isolated from wild penguins. . Int J Syst Evol Microbiol 53, 10091012. [View Article] [PubMed]
    [Google Scholar]
  20. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  21. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  22. Kim P. S., Shin N. R., Kim J. Y., Yun J. H., Hyun D. W., Bae J. W. ( 2013 ). Gibbsiella papilionis sp. nov., isolated from the intestinal tract of the butterfly Mycalesis gotama, and emended description of the genus Gibbsiella . . Int J Syst Evol Microbiol 63, 26072611. [View Article] [PubMed]
    [Google Scholar]
  23. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [View Article]
    [Google Scholar]
  24. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  25. Lehmann K. B., Neumann R. ( 1896 ). Atlas und Grundriss der Bakteriologie und Lehrbuch der Speziellen Bakteriologischen Diagnostik. Munich:: J. F. Lehmann;.
    [Google Scholar]
  26. Loy A., Schulz C., Lücker S., Schöpfer-Wendels A., Stoecker K., Baranyi C., Lehner A., Wagner M. ( 2005 ). 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales”. . Appl Environ Microbiol 71, 13731386. [View Article] [PubMed]
    [Google Scholar]
  27. MIDI ( 1999 ). Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  28. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. ( 1992 ). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett 100, 5965. [View Article] [PubMed]
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  30. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI;.
    [Google Scholar]
  31. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  32. Schön R., Groth I. ( 2006 ). Practical thin layer chromatography techniques for diaminopimelic acid and whole cell sugar analyses in the classification of environmental actinomycetes. . J Basic Microbiol 46, 243249. [View Article] [PubMed]
    [Google Scholar]
  33. Shin N. R., Jung M. J., Kim M. S., Roh S. W., Nam Y. D., Bae J. W. ( 2011 ). Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter. . Int J Syst Evol Microbiol 61, 24302434. [View Article] [PubMed]
    [Google Scholar]
  34. Sjödén B., Funke G., Izquierdo A., Akervall E., Collins M. D. ( 1998 ). Description of some coryneform bacteria isolated from human clinical specimens as Corynebacterium falsenii sp. nov.. Int J Syst Bacteriol 48, 6974. [View Article] [PubMed]
    [Google Scholar]
  35. Taguchi R., Houjou T., Nakanishi H., Yamazaki T., Ishida M., Imagawa M., Shimizu T. ( 2005 ). Focused lipidomics by tandem mass spectrometry. . J Chromatogr B Analyt Technol Biomed Life Sci 823, 2636. [View Article] [PubMed]
    [Google Scholar]
  36. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  37. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  38. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  39. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575580.[PubMed]
    [Google Scholar]
  40. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  41. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. ( 2000 ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. . Int J Syst Evol Microbiol 50, 12971303. [View Article] [PubMed]
    [Google Scholar]
  42. Yano I., Saito K. ( 1972 ). Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans . . FEBS Lett 23, 352356. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.067587-0
Loading
/content/journal/ijsem/10.1099/ijs.0.067587-0
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error