1887

Abstract

A Gram-stain-negative, non-flagellated, aerobic and ovoid or rod-shaped bacterium, designated J-MS1, was isolated from seashore sand in the South Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain J-MS1 was found to grow optimally at 30 °C and pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain J-MS1 belonged to the genus , clustering coherently with the type strain of and sharing 98.34 % sequence similarity. Strain J-MS1 exhibited 16S rRNA gene sequence similarity of 94.26–96.98 % to the type strains of the other species of the genus . In the phylogenetic trees based on sequences, strain J-MS1 clustered with the type strain of , with which it shared the highest sequence similarity (86.97 %). Strain J-MS1 contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), C and Cω7 as the major fatty acids. The major polar lipids detected in strain J-MS1 and in the type strain of were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain J-MS1 was 49.8 mol% and its mean DNA–DNA relatedness value with LMG 23818 was 12 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain J-MS1 is separated from recognized species of the genus . On the basis of the data presented, strain J-MS1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J-MS1 ( = KCTC 42112 = CECT 8623).

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2014-11-01
2019-11-22
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