1887

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated RA1, was isolated from faeces collected from Beluga whale () in Yeosu aquarium, South Korea. Strain RA1 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1 joins the cluster comprising the type strains of three species of the genus , with which it exhibited 95.8–96.0 % sequence similarity. Sequence similarities to the type strains of other recognized species were less than 94.3 %. Strain RA1 contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), Cω7 and C as the major fatty acids. The major polar lipids of strain RA1 were phosphatidylethanolamine, phosphatidylglycerol, two unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain RA1 was 47.4 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain RA1 is separated from other species of the genus . On the basis of the data presented, strain RA1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RA1 ( = KCTC 42154 = NBRC 110551).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.067405-0
2014-12-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/4068.html?itemId=/content/journal/ijsem/10.1099/ijs.0.067405-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L.. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y.. ( 1957;). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef]
    [Google Scholar]
  5. Embley T. M., Wait R.. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G... Chichester:: Wiley;.
    [Google Scholar]
  6. Gärtner A., Wiese J., Imhoff J. F.. ( 2008;). Amphritea atlantica gen. nov., sp. nov., a gammaproteobacterium from the Logatchev hydrothermal vent field. . Int J Syst Evol Microbiol 58:, 34–39. [CrossRef][PubMed]
    [Google Scholar]
  7. Kim Y.-O., Park S., Nam B.-H., Jung Y.-T., Kim D.-G., Lee J.-S., Lee S.-J., Yoon J.-H.. ( 2013;). Litoreibacter halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. . Int J Syst Evol Microbiol 63:, 3364–3368. [CrossRef][PubMed]
    [Google Scholar]
  8. Komagata K., Suzuki K.. ( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  9. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  10. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  11. Miyazaki M., Nogi Y., Fujiwara Y., Kawato M., Nagahama T., Kubokawa K., Horikoshi K.. ( 2008;). Amphritea japonica sp. nov. and Amphritea balenae sp. nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan. . Int J Syst Evol Microbiol 58:, 2815–2820. [CrossRef][PubMed]
    [Google Scholar]
  12. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  13. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  14. Staley J. T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  15. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H.. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53:, 53–57. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.067405-0
Loading
/content/journal/ijsem/10.1099/ijs.0.067405-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error