1887

Abstract

A Gram-stain negative, facultatively anaerobic, non-motile, rod-shaped bacterium, designated strain THG-A13, was isolated from rhizosphere soil in Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence comparisons, strain THG-A13 had close similarity with GH34-4 (98.5 %), YC6269 (97.9 %) and GH19-3 (97.3 %). Chemotaxonomic data revealed that strain THG-A13 possesses ubiquinone-8 (Q8) as the predominant isoprenoid quinone and iso-C, iso-C and iso-Cω9 as the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol) and diphosphatidylglycerol. The G+C content was 66.3 mol%. The DNA–DNA relatedness values between strain THG-A13 and its closest phylogenetic neighbours were below 18.0 %. These data corroborated the affiliation of strain THG-A13 to the genus . These data suggest that the isolate represents a novel species for which the name sp. nov. is proposed, with THG-A13 as the type strain ( = KACC 17646 = JCM 19613).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.067397-0
2015-02-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/2/587.html?itemId=/content/journal/ijsem/10.1099/ijs.0.067397-0&mimeType=html&fmt=ahah

References

  1. Aslam Z., Yasir M., Jeon C. O., Chung Y. R.. ( 2009;). Lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.). . Int J Syst Evol Microbiol 59:, 675–680. [CrossRef][PubMed]
    [Google Scholar]
  2. Christensen P., Cook F. D.. ( 1978;). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. . Int J Syst Bacteriol 28:, 367–393. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Jones D.. ( 1981;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45:, 316–354.[PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Fautz E., Reichenbach H.. ( 1980;). A simple test for flexirubin-type pigments. . FEMS Microbiol Ecol 8:, 87–91. [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucl Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  8. Hashizume H., Hattori S., Igarashi M., Akamatsu Y.. ( 2004;). Tripropeptin E, a new tripropeptin group antibiotic produced by Lysobacter sp. BMK333-48F3. . J Antibiot (Tokyo) 57:, 394–399. [CrossRef][PubMed]
    [Google Scholar]
  9. Hiraishi A., Ueda Y., Ishihara J., Mori T.. ( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high–performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42:, 457–469. [CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  12. Kumar S., Nei M., Dudley J., Tamura K.. ( 2008;). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9:, 299–306. [CrossRef][PubMed]
    [Google Scholar]
  13. Lee J. W., Im W. T., Kim M. K., Yang D. C.. ( 2006;). Lysobacter koreensis sp. nov., isolated from a ginseng field. . Int J Syst Evol Microbiol 56:, 231–235. [CrossRef][PubMed]
    [Google Scholar]
  14. Luo G., Shi Z., Wang G.. ( 2012;). Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. . Int J Syst Evol Microbiol 62:, 1659–1665. [CrossRef][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parleet J. H.. ( 1984;). An intergrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Moore D. D., Dowhan D.. ( 1995;). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K... New York:: Wiley;.
    [Google Scholar]
  19. Park J. H., Kim R., Aslam Z., Jeon C. O., Chung Y. R.. ( 2008;). Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter. . Int J Syst Evol Microbiol 58:, 387–392. [CrossRef][PubMed]
    [Google Scholar]
  20. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  21. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  22. Tamaoka J., Katayama-Fujiruma Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  24. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  26. Wei D. Q., Yu T. T., Yao J. C., Zhou E. M., Song Z. Q., Yin Y.-R., Ming H., Tang S.-K., Li W.-J.. ( 2012;). Lysobacter thermophilus sp. nov., isolated from a geothermal soil sample in Tengchong, south-west China. . Antonie van Leeuwenhoek 102:, 643–651. [CrossRef][PubMed]
    [Google Scholar]
  27. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  28. Weon H. Y., Kim B. Y., Baek Y. K., Yoo S. H., Kwon S. W., Stackebrandt E., Go S. J.. ( 2006;). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. . Int J Syst Evol Microbiol 56:, 947–951. [CrossRef][PubMed]
    [Google Scholar]
  29. Weon H. Y., Kim B. Y., Kim M. K., Yoo S. H., Kwon S. W., Go S. J., Stackebrandt E.. ( 2007;). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 57:, 548–551. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.067397-0
Loading
/content/journal/ijsem/10.1099/ijs.0.067397-0
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error