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Volume 64, Issue Pt_11

sp. nov., isolated from seashore sand Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, gliding and rod-shaped bacterial strain, designated GJMS-9, was isolated from seashore sand collected at Geoje island in the South Sea, South Korea. Strain GJMS-9 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-9 clustered with the type strain of , showing the highest sequence similarity of 99.1 %. Strain GJMS-9 exhibited 16S rRNA gene sequence similarity of 96.5 % to the type strain of and of less than 96.1 % to the type strains of other recognized species. Strain GJMS-9 contained MK-6 as the only menaquinone and iso-C G, iso-C 3-OH, iso-C and iso-C 3-OH as the major fatty acids. The polar lipid profile of strain GJMS-9 containing phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid as major components was similar to that of the type strain of . The DNA G+C content of strain GJMS-9 was 32.2 mol% and its DNA–DNA relatedness with DSM 18436 was 38±6.1 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJMS-9 is separated from other species of the genus . On the basis of the data presented, strain GJMS-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GJMS-9 ( = KCTC 42117 = CECT 8597).

  • Published Online:
Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE)
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award NRF-2013M3A9A5075953)
© 2014 IUMS
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2014-11-01
2024-12-04
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References

  1. Asker D., Beppu T., Ueda K. ( 2007 ). Mesoflavibacter zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae . . Syst Appl Microbiol 30, 291–296. [View Article] [PubMed]
    [Google Scholar]
  2. Asker D., Beppu T., Ueda K. ( 2008 ). Mesoflavibacter zeaxanthinifaciens gen. nov., sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 122. . Int J Syst Evol Microbiol 58, 1511–1512. [View Article] [PubMed]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  4. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
     [Google Scholar]
  5. Bernardet J.-F. ( 2011 ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N. L., Brown D. R., Parte A. . New York:: Springer;.
     [Google Scholar]
  6. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  7. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  8. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  9. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 25–68. [View Article]
    [Google Scholar]
  10. Embley T. M., Wait R. ( 1994 ). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
     [Google Scholar]
  11. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  12. Komagata K., Suzuki K.-I. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  13. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  14. Lee J. H., Hwang Y. M., Baik K. S., Choi K. S., Ka J.-O., Seong C. N. ( 2014 ). Mesoflavibacter aestuarii sp. nov., a zeaxanthin-producing marine bacterium isolated from seawater. . Int J Syst Evol Microbiol 64, 1932–1937. [View Article] [PubMed]
    [Google Scholar]
  15. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184.[PubMed]
    [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  17. Park S., Jung Y.-T., Yoon J.-H. ( 2014 ). Kordia jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Kordia . . Int J Syst Evol Microbiol 64, 657–662. [View Article] [PubMed]
    [Google Scholar]
  18. Peña A., Valens M., Santos F., Buczolits S., Antón J., Kämpfer P., Busse H.-J., Amann R., Rosselló-Mora R. ( 2005 ). Intraspecific comparative analysis of the species Salinibacter ruber . . Extremophiles 9, 151–161. [View Article] [PubMed]
    [Google Scholar]
  19. Reichenbach H. ( 1992 ). The order Cytophagales . . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., vol. 4, pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;.
     [Google Scholar]
  20. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  21. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  22. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 1921–1942.[PubMed]
    [Google Scholar]
  23. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  25. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502–505. [View Article]
    [Google Scholar]
  26. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187–194. [View Article] [PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 53–57. [View Article] [PubMed]
    [Google Scholar]
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Mesoflavibacter sabulilitoris sp. nov., isolated from seashore sand
Int J Syst Evol Microbiol 64, 3743 (2014); https://doi.org/10.1099/ijs.0.067223-0
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