1887

Abstract

A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002, was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and translucent white. The ranges of temperature, pH and salt concentration for growth were 8–30 °C, pH 6.0–10.0 and 1–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-002 belongs to the genus of the class . Levels of similarity between the 16S rRNA gene sequence of strain TAR-002 and those of the type strains of species of the genus were 93.5–98.9 %; the most closely related species was . In DNA–DNA hybridization assays between strain TAR-002 and its phylogenetic neighbours, DS-18, J22, ATCC 15264 and DSM 4736, mean hybridization levels were 6.4–27.7 %. The G+C content of strain TAR-002 was 70.3 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were Cω7 and C, and the presence of 1,2-di--acyl-3--[-glucopyranosyl-(1→4)-α--glucopyranuronosyl]glycerol (DGL) indicates the affiliation of strain TAR-002 with the genus . On the basis of biological characteristics and 16S rRNA gene sequence comparisons, strain TAR-002 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is TAR-002 ( = NBRC 110107 = CECT 8537).

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2014-11-01
2019-11-13
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